In order to measure the grating constants of different types of holographic samples efficiently and conveniently, we use spectrophotometers and the macro angle-resolved spectrum system to measure spectral information from six different holographic samples with three types, including rainbow holographic samples with light pillars, plain rainbow holographic samples, and matte holographic samples with light pillars. The information obtained from the instruments was used to investigate their grating constants by the grating equation. It was found that for the grating constant calculation, compared with the values measured using a light optical microscope, the angle-resolved spectrum system and the 45/0 spectrophotometers were applicable to the samples with rainbow effects. For the matte holographic samples, the angle-resolved spectrum system, which was mainly used to distinguish the differences between different matte samples, was necessary. The results from the spectrometric methods were similar to those from the light optical microscope.