Boronate-Based Probes for Biological Oxidants: A Novel Class of Molecular Tools for Redox Biology

Adam Sikora; Jacek Zielonka; Karolina Dębowska; Radosław Michalski; Renata Smulik-Izydorczyk; Jakub Pięta; Radosław Podsiadły; Angelika Artelska; Karolina Pierzchała; Balaraman Kalyanaraman

doi:10.3389/fchem.2020.580899

Boronate-Based Probes for Biological Oxidants: A Novel Class of Molecular Tools for Redox Biology

Front Chem. 2020 Sep 25:8:580899. doi: 10.3389/fchem.2020.580899. eCollection 2020.

Affiliations

¹ Faculty of Chemistry, Institute of Applied Radiation Chemistry, Lodz University of Technology, Lodz, Poland.
² Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI, United States.
³ Faculty of Chemistry, Institute of Polymer and Dye Technology, Lodz University of Technology, Lodz, Poland.

Abstract

Boronate-based molecular probes are emerging as one of the most effective tools for detection and quantitation of peroxynitrite and hydroperoxides. This review discusses the chemical reactivity of boronate compounds in the context of their use for detection of biological oxidants, and presents examples of the practical use of those probes in selected chemical, enzymatic, and biological systems. The particular reactivity of boronates toward nucleophilic oxidants makes them a distinct class of probes for redox biology studies. We focus on the recent progress in the design and application of boronate-based probes in redox studies and perspectives for further developments.

Keywords: NADPH oxidases; azanone; boronic acids; hydrogen peroxide; hydroperoxides; hypochlorous acid; molecular probes; peroxynitrite.

Publication types

Review