Antioxidant and cytotoxic activities of the ethyl acetate extract of Sphagneticola trilobata (L.) J.F. Pruski on MCF-7 breast cancer cell

Vivi Mardina; Syafruddin Ilyas; Tisna Harmawan; Halimatussakdiah Halimatussakdiah; Masitta Tanjung

doi:10.4103/japtr.JAPTR_31_20

Antioxidant and cytotoxic activities of the ethyl acetate extract of Sphagneticola trilobata (L.) J.F. Pruski on MCF-7 breast cancer cell

J Adv Pharm Technol Res. 2020 Jul-Sep;11(3):123-127. doi: 10.4103/japtr.JAPTR_31_20. Epub 2020 Jul 14.

Authors

Vivi Mardina¹, Syafruddin Ilyas², Tisna Harmawan³, Halimatussakdiah Halimatussakdiah³, Masitta Tanjung²

Affiliations

¹ Department of Biology, Samudra University, Kampus Unsam Meurandeh, Langsa, Indonesia.
² Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia.
³ Department of Chemistry, Faculty of Engineering, Samudra University, Kampus Unsam Meurandeh, Langsa, Indonesia.

Abstract

Sphagneticola trilobata (L.) J.F. Pruski is the perennial herb distributed at tropical temperature. In this study, the antioxidant and anticancer properties of the ethyl acetate extract from S. trilobata leaves were investigated against MCF-7 breast cancer cells. The antioxidant and anticancer activities were assessed by 1,1-diphenyl-2-picrylhydrazyl free radical scavenging and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay methods, respectively. The extract demonstrated DPPH free radical scavenging effect with IC₅₀ value equaling to 127.43 μg/mL. The cytotoxic study was conducted on the concentration range of 1-200 μg/mL, and the results exhibited the strongest inhibitory activity against MCF-7 with the LC₅₀ value of 58.143 μg/mL. The cytotoxic activity of the extract was supported by the induction of apoptosis cell which possessed the apoptosis percentage of 78.80%. Thus, the cheap herbal drug treatment might highly be recommended to treat effectively breast cancers as an ideal choice or combinational therapy.

Keywords: Antioxidant; MCF-7; Sphagneticola trilobata; cytotoxicity.