Structural insights into Pseudomonas aeruginosaType six secretion system exported effector 8

J Struct Biol. 2020 Dec 1;212(3):107651. doi: 10.1016/j.jsb.2020.107651. Epub 2020 Oct 20.

Abstract

Recent reports indicate that the Type six secretion system exported effector 8 (Tse8) is a cytoactive effector secreted by the Type VI secretion system (T6SS) of the human pathogen Pseudomonas aeruginosa. The T6SS is a nanomachine that assembles inside of the bacteria and injects effectors/toxins into target cells, providing a fitness advantage over competing bacteria and facilitating host colonisation. Here we present the first crystal structure of Tse8 revealing that it conserves the architecture of the catalytic triad Lys84-transSer162-Ser186 that characterises members of the Amidase Signature superfamily. Furthermore, using binding affinity experiments, we show that the interaction of phenylmethylsulfonyl fluoride (PMSF) to Tse8 is dependent on the putative catalytic residue Ser186, providing support for its nucleophilic reactivity. This work thus demonstrates that Tse8 belongs to the Amidase Signature (AS) superfamily. Furthermore, it highlights Tse8 similarity to two family members: the Stenotrophomonas maltophilia Peptide Amidase and the Glutamyl-tRNAGln amidotransferase subunit A from Staphylococcus aureus.

Keywords: Bacterial secretion; Protein export; Pseudomonas aeruginosa; Structural biology; Type VI Secretion System.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amidohydrolases / chemistry
  • Bacterial Proteins / chemistry*
  • Bacterial Secretion Systems / chemistry*
  • Pseudomonas aeruginosa / chemistry*
  • RNA, Transfer / chemistry
  • Type VI Secretion Systems / chemistry*

Substances

  • Bacterial Proteins
  • Bacterial Secretion Systems
  • Type VI Secretion Systems
  • RNA, Transfer
  • Amidohydrolases
  • amidase