Vincristine and prednisone regulates cellular and exosomal miR-181a expression differently within the first time diagnosed and the relapsed leukemia B cells

Shabirul Haque; Sarah R Vaiselbuh

doi:10.1016/j.lrr.2020.100221

Vincristine and prednisone regulates cellular and exosomal miR-181a expression differently within the first time diagnosed and the relapsed leukemia B cells

Leuk Res Rep. 2020 Sep 28:14:100221. doi: 10.1016/j.lrr.2020.100221. eCollection 2020.

Authors

Shabirul Haque¹, Sarah R Vaiselbuh^{1

2}

Affiliations

¹ The Feinstein Institute for Medical Research, Northwell Health, 350 Community Drive, Manhasset, New York 11030, USA.
² Department of Pediatrics, Staten Island University Hospital, Northwell Health, 475 Seaview Ave, Staten Island, New York, 10305, USA.

Abstract

We explored the effect of vincristine and prednisone on cellular and exosomal miR-181a expression in first time diagnosed leukemia and relapsed leukemia. Vincristine and prednisone induced apoptosis/pro-apoptotic genes in first time diagnosed leukemia, and suppressed the cellular and exosomal miR-181a expression. In contrast, vincristine and prednisone could not induce apoptosis/pro-apoptotic genes in relapsed leukemia, and could not change the expression of cellular or exosomal miR-181a. In conclusion, the non-suppressive nature of miR-181a in relapsed leukemia might contribute to the chemo-resistance and this suggests a potential role of miR-181a-inhibitor along with the chemotherapy in the treatment of relapsed leukemia.

Keywords: Abbreviations: Cell-miR-181a, cellular expression of miR-181a; Acute lymphocytic leukemia; Exo-miR-181a, exosomal expression of miR-181a; Exosomes; miR-181a, relapsed leukemia.