The emergence of a novel recombinant porcine reproductive and respiratory syndrome virus with an amino acid insertion in GP5 protein

Zhongbao Song; Qiaoya Zhang; Ye Chen; Haiyang Shen; Guang Yang; Ping Jiang; Ji-Long Chen; Li Lin

doi:10.1016/j.micpath.2020.104573

The emergence of a novel recombinant porcine reproductive and respiratory syndrome virus with an amino acid insertion in GP5 protein

Microb Pathog. 2020 Dec:149:104573. doi: 10.1016/j.micpath.2020.104573. Epub 2020 Oct 19.

Authors

Zhongbao Song¹, Qiaoya Zhang², Ye Chen³, Haiyang Shen⁴, Guang Yang⁴, Ping Jiang⁵, Ji-Long Chen³, Li Lin⁶

Affiliations

¹ Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China. Electronic address: virus_szb@163.com.
² College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, Shandong, China.
³ Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
⁴ College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, China.
⁵ Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
⁶ College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, China. Electronic address: linli@zhku.edu.cn.

PMID: 33091580
DOI: 10.1016/j.micpath.2020.104573

Abstract

As an economic devastating virus, porcine reproductive and respiratory syndrome virus (PRRSV) has spread globally, and seriously hinders the healthy development of the swine industry worldwide. In recent years, however, recombinant PRRSV strains are continuously emerging, resulting in the death of a large number of pigs in China. In this study, we reported a NADC30-like PRRSV strain GD1909, a recombinant virus, which may originate from NADC30-like and HUN4-like strains. The GP5 protein of GD1909 strain has an asparagine insertion at position 60 and has more complex glycosylation pattern. This should be helpful for a better understanding of PRRSV molecular epidemiology and the prevention of PRRSV infection in the future.

Keywords: GP5; Insertion; NADC30-Like; PRRSV; Recombination.

MeSH terms

Amino Acids
Animals
China
Genome, Viral
Phylogeny
Porcine Reproductive and Respiratory Syndrome*
Porcine respiratory and reproductive syndrome virus* / genetics
Swine

Substances

Amino Acids