Immunoglobulin Receptors Expression in Indian Colon Cancer Patients and Healthy Subjects Using a Noninvasive Approach and Flowcytometry

Rama Chaudhry; Vishwa Deepak Bamola; Projoyita Samanta; Divya Dubey; Tej Bahadur; Monica Chandan; Shyam Tiwary; Abhipray Gahlowt; Neha Nair; Harneet Kaur; Chena Passi; Atul Sharma; Dinesh S Chandel; Pinaki Panigrahi

doi:10.4103/ijabmr.IJABMR_191_19

Immunoglobulin Receptors Expression in Indian Colon Cancer Patients and Healthy Subjects Using a Noninvasive Approach and Flowcytometry

Int J Appl Basic Med Res. 2020 Jul-Sep;10(3):194-199. doi: 10.4103/ijabmr.IJABMR_191_19. Epub 2020 Jul 11.

Authors

Affiliations

¹ Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India.
² Department of Medical Oncology, All India Institute of Medical Sciences, New Delhi, India.
³ Department of Environmental, Agricultural and Occupational Health, Center for Global Health and Development, College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska, USA.
⁴ Department of Epidemiology, Center for Global Health and Development, College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska, USA.
⁵ Department of Pediatrics, Center for Global Health and Development, College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska, USA.

Abstract

Background: Isolation of viable colonocytes from human stool is a noninvasive and convenient approach that can be used for diagnostic, screening, management, and research on various gastrointestinal (GI) diseases including colon cancer. Limited studies are available globally and for the first time in this article, we have reported the immunoglobulin (Ig) (IgA and IgG) receptors concentration on viable colonocytes for Indian colon cancer patients using this noninvasive approach.

Materials and methods: Viable colonocytes from stool were isolated by the Somatic Cell Sampling and Recovery method (Noninvasive Technology, USA) and processed for the assessment of Igs (IgA and IgG) receptors expression using standard immunophenotyping and flow cytometry.

Results: IgA and IgG receptor expression was measured and reported on these viable colonocytes. There was a significant difference in the expression of IgA and IgG receptors on viable colonocytes between colon cancer patients and healthy individuals.

Conclusion: This noninvasive technique is a promising approach for the detection of molecular and immunological markers that will help clinicians in the diagnosis, screening, monitoring, and management of different GI diseases including colon cancer.

Keywords: Colon cancer; colonocytes; flowcytometry; immunoglobulins; noninvasive.