ssc-miR-451 Regulates Porcine Primary Adipocyte Differentiation by Targeting ACACA

Mailin Gan; Linyuan Shen; Yuan Fan; Ya Tan; Lin Liu; Lei Chen; Ye Zhao; Lili Niu; Guoqing Tang; Qiang Li; Xu Xu; Tinghuan Zhang; Xuewei Li; Shunhua Zhang; Li Zhu

doi:10.3390/ani10101891

ssc-miR-451 Regulates Porcine Primary Adipocyte Differentiation by Targeting ACACA

Animals (Basel). 2020 Oct 16;10(10):1891. doi: 10.3390/ani10101891.

Authors

Mailin Gan^{1

2}, Linyuan Shen^{1

2}, Yuan Fan^{1

2}, Ya Tan^{1

2

3}, Lin Liu^{1

2}, Lei Chen^{1

2}, Ye Zhao^{1

2}, Lili Niu^{1

2}, Guoqing Tang^{1

2}, Qiang Li⁴, Xu Xu⁴, Tinghuan Zhang⁵, Xuewei Li^{1

2}, Shunhua Zhang^{1

2}, Li Zhu^{1

2}

Affiliations

¹ College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
² Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.
³ Institute of Animal Husbandry and Veterinary, Guizhou Academy of Agricultural Science, Guiyang 550005, China.
⁴ Sichuan Province General Station of Animal Husbandry, Chengdu 611130, China.
⁵ Chongqing Academy of Animal Science, Rongchang County, Chongqing 402460, China.

Abstract

miRNA is a small non-coding RNA, which plays an important role in diverse biological processes. In the present study, we explore the effect of ssc-miR-451 on porcine adipose development and meat quality. We observed that ssc-miR-451 was downregulated during porcine primary adipocyte differentiation. Overexpression of ssc-miR-451 inhibited adipogenic differentiation, while inhibition of ssc-miR-451 promoted adipogenic differentiation. The dual luciferase reporter system indicated Acetyl-CoA carboxylase alpha (ACACA) as a target gene of ssc-miR-451. Correlation analysis negatively correlated miR-451 expression with intramuscular fat content (IMF) and positively correlated ACACA expression with IMF. Further analysis of fatty acid composition revealed that pigs with high expression of ssc-miR-451 had higher monounsaturated fatty acid (MUFA) and lower polyunsaturated fatty acid (PUFA). Taken together, our study suggests that ssc-miR-451 regulates lipid deposition and fatty acid composition by targeting ACACA, and ssc-miR-451 may serve as a potential genetic marker to improve pork quality.

Keywords: ACACA; adipocyte differentiation; fatty acid; meat quality; ssc-miR-451.

Abstract

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