Non-covalent binding and selective fluorescent sensing of dipyrone with a carbocyanine dye and cetyltrimethylammonium bromide

Sofia A Zakharenkova; Anna S Kriveleva; Ekaterina A Katkova; Irina A Doroshenko; Nikolai Yu Polovkov; Tatyana A Podrugina; Mikhail K Beklemishev

doi:10.1088/2050-6120/abc13c

Non-covalent binding and selective fluorescent sensing of dipyrone with a carbocyanine dye and cetyltrimethylammonium bromide

Methods Appl Fluoresc. 2021 Jan 11;9(1). doi: 10.1088/2050-6120/abc13c.

Authors

Sofia A Zakharenkova¹, Anna S Kriveleva¹, Ekaterina A Katkova¹, Irina A Doroshenko¹, Nikolai Yu Polovkov², Tatyana A Podrugina¹, Mikhail K Beklemishev¹

Affiliations

¹ Lomonosov Moscow State University, Department of Chemistry, 119991 GSP-1, Moscow, Russia.
² Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Leninsky Prospect 29, 119991 Moscow, Russia.

PMID: 33053517
DOI: 10.1088/2050-6120/abc13c

Abstract

The work is aimed at the search of selective fluorescent sensors without using specific artificial receptors, antibodies, enzymes etc. With this end in view, methods based on non-covalent binding of target analytes are sought. We observed dramatic changes in the emission spectrum of a carbocyanine dye in a micellar surfactant solution (cetyltrimethylammonium bromide, CTAB) in the presence of dipyrone (metamizol, analgin): the 480 nm band intensity increases with a simultaneous decrease in intensity in near-IR region (720 nm). MALDI and NMR-¹H data show the intact molecules of dipyrone and the dye. The detection can be performed in the presence of other organic species and inorganic salts. Dipyrone testing is feasible within 5 × 10^-7-5 × 10^-4M with RSDs of 3.5% by using a visualizer instead of a spectrofluorimeter.

Keywords: carbocyanine; cetyltrimethylammonium; dipyrone; fluorescent sensing; non-covalent binding; ratiometric signal.