Translation-coupled RNA replication and parasitic replicators in membrane-free compartments

Ryo Mizuuchi; Norikazu Ichihashi

doi:10.1039/d0cc06606k

Translation-coupled RNA replication and parasitic replicators in membrane-free compartments

Chem Commun (Camb). 2020 Nov 3;56(87):13453-13456. doi: 10.1039/d0cc06606k.

Authors

Ryo Mizuuchi¹, Norikazu Ichihashi²

Affiliations

¹ Komaba Institute for Science, The University of Tokyo, Meguro, Tokyo 153-8902, Japan. mizuuchi@bio.c.u-tokyo.ac.jp and JST, PRESTO, Kawaguchi, Saitama 332-0012, Japan.
² Komaba Institute for Science, The University of Tokyo, Meguro, Tokyo 153-8902, Japan. mizuuchi@bio.c.u-tokyo.ac.jp and Department of Life Science, Graduate School of Arts and Science, The University of Tokyo, Meguro, Tokyo 153-8902, Japan and Universal Biology Institute, The University of Tokyo, Meguro, Tokyo 153-8902, Japan.

PMID: 33043949
DOI: 10.1039/d0cc06606k

Abstract

We report RNA self-replication through the translation of its encoded protein within membrane-free compartments generated by liquid-liquid phase separation. The aqueous droplets support RNA self-replication by concentrating a genomic RNA and translation proteins, facilitating the uptake of small substrates, and preventing the replication of parasitic RNAs through compartmentalization.

MeSH terms

Protein Biosynthesis*
RNA / biosynthesis*
RNA / genetics*

Substances

RNA