Effect of methoxyflavones contained in Kaempferia parviflora on CRE-mediated transcription in PC12D cells

Noriyuki Natsume; Aki Yamano; Akio Watanabe; Takayuki Yonezawa; Je-Tae Woo; Tohru Yamakuni; Toshiaki Teruya

doi:10.1016/j.bmcl.2020.127606

Effect of methoxyflavones contained in Kaempferia parviflora on CRE-mediated transcription in PC12D cells

Bioorg Med Chem Lett. 2020 Dec 1;30(23):127606. doi: 10.1016/j.bmcl.2020.127606. Epub 2020 Oct 7.

Authors

Noriyuki Natsume¹, Aki Yamano², Akio Watanabe³, Takayuki Yonezawa³, Je-Tae Woo⁴, Tohru Yamakuni⁵, Toshiaki Teruya⁶

Affiliations

¹ Graduate School of Engineering and Science, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, Japan.
² Faculty of Education, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, Japan.
³ Research Institute for Biological Functions, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan.
⁴ Department of Biological Chemistry, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan.
⁵ Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan; Research Center of Supercritical Fluid Technology, Graduate School of Engineering, Tohoku University, 6-6-07 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan; New Industry Creation Hatchery Center, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan; Graduate School of Bioresources and Environmental Sciences, Ishikawa Prefectural University, 1-308, Suematsu, Nonoichi-shi, Ishikawa-ken 921-8836, Japan.
⁶ Faculty of Education, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, Japan. Electronic address: t-teruya@edu.u-ryukyu.ac.jp.

PMID: 33038547
DOI: 10.1016/j.bmcl.2020.127606

Abstract

The cAMP-response element (CRE) is critical in the formation of long-term memory. To prove the pharmacological effects of the methoxyflavones-rich residue (MRR) and its constituent methoxyflavones (1-9) extracted from the rhizomes of Kaempferia parviflora on the nervous system, we examined the effects of the MRR and methoxyflavones (1-9) on CRE-mediated transcription in PC12D cells. The MRR increased CRE-mediated transcription in PC12D cells. In addition, among methoxyflavones (1-9) isolated from MRR, compounds 1-4 increased CRE-mediated transcription. These results suggest that K. parviflora and methoxyflavone might be very useful materials for preventing and recovering from cognitive decline.

Keywords: Alzheimer’s disease; CRE-mediated transcription; Kaempferia parviflora; Methoxyflavones; PC12D cells.

MeSH terms

Animals
Cell Survival / drug effects
Flavones / isolation & purification
Flavones / pharmacology*
Flavones / toxicity
Molecular Structure
PC12 Cells
Plant Extracts / isolation & purification
Plant Extracts / pharmacology
Plant Extracts / toxicity
Rats
Response Elements / physiology
Structure-Activity Relationship
Transcription, Genetic / drug effects*
Zingiberaceae / chemistry*

Substances

Flavones
Plant Extracts