Novosphingobium aquimarinum sp. nov., isolated from seawater

Abstract

A novel Gram-stain-negative, aerobic, and rod-shaped bacterial strain, M24A2M^T, was isolated from seawater in the Republic of Korea. On the basis of the 16S rRNA gene phylogeny, strain M24A2M^T was found to be closely related to Novosphingobium pentaromativorans US6-1^T and Novosphingobium mathurense SM117^T with pair-wise sequence similarities of 97.4 and 96.9 %, respectively. Phylogenetic analysis of 16S rRNA sequences indicated that M24A2M^T formed a branch with Novosphingobium pentaromativorans US6-1^T and represented a member of the genus Novosphingobium. The predominant cellular fatty acids were C_{14 : 0} 2-OH, summed feature 3 (C_{16 : 1} ω7c and/or C_{16 : 1} ω6c), and summed feature 8 (C_{18 : 1} ω7c and/or C_{18 : 1} ω6c). The polar lipids of strain M24A2M^T consisted mainly of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one unidentified phospholipid, and two unidentified lipids. The respiratory quinone was ubiquinone Q-10. The genomic DNA G+C content was 63.9 %. Given the phenotypic characteristics along with the phylogenetic distinctness and chemotaxonomic features, strain M24A2M^T is considered to represent a novel species within the genus Novosphingobium, for which the name Novosphingobium aquimarinum sp. nov. is proposed. The type strain of Novosphingobium aquimarinum sp. nov. is M24A2M^T (=KCTC 72894^T=JCM 33983^T).

Keywords: Novosphingobium aquimarinum; polyphasic characterization; seawater.