Identification of peptides in blood following oral administration of β-conglycinin to Wistar rats

Xiaojing Sheng; Satoshi Nagaoka; Marika Hashimoto; Yusuke Amiya; Masato Beppu; Kenichiro Tsukamoto; Emiko Yanase; Mitsuru Tanaka; Toshiro Matsui

doi:10.1016/j.foodchem.2020.128197

Identification of peptides in blood following oral administration of β-conglycinin to Wistar rats

Food Chem. 2021 Mar 30;341(Pt 1):128197. doi: 10.1016/j.foodchem.2020.128197. Epub 2020 Sep 28.

Authors

Xiaojing Sheng¹, Satoshi Nagaoka², Marika Hashimoto¹, Yusuke Amiya¹, Masato Beppu², Kenichiro Tsukamoto², Emiko Yanase², Mitsuru Tanaka³, Toshiro Matsui⁴

Affiliations

¹ Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
² Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
³ Research and Development Center for Five-Sense Devices, Kyushu University, Fukuoka 819-0395, Japan.
⁴ Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan; Research and Development Center for Five-Sense Devices, Kyushu University, Fukuoka 819-0395, Japan. Electronic address: tmatsui@agr.kyushu-u.ac.jp.

PMID: 33032251
DOI: 10.1016/j.foodchem.2020.128197

Abstract

In this study, β-conglycinin (100 mg/kg) was orally administered to Wistar rats in order to identify peptides that may be derived from the protein in the blood. Plasma samples taken from the tail vein up to 8 h after administration were analyzed by matrix-assisted laser desorption/ionization (MALDI) and liquid chromatography-time-of-flight (LC-TOF) mass spectrometry (MS). In total, 126 signals were detected by MALDI-MS. Among the signals, nine oligopeptides (SEL, KGPL, SILGA, DSEL, GDANI, SYFV, CLQSC, GEQPRPF, and LVINEGDA) were successfully identified as β-conglycinin-derived peptides by LC-TOF/MS at a plasma concentration of 0.75-756 pmol/mL. The results demonstrated that β-conglycinin could be the dietary source protein for the oligopeptides produced prior to entering the circulating bloodstream of rats.

Keywords: Blood peptides; LC-TOF/MS; MALDI-MS; Wistar rats; β-Conglycinin.

MeSH terms

Administration, Oral
Animals
Antigens, Plant / administration & dosage*
Antigens, Plant / blood*
Chromatography, Liquid / methods
Globulins / administration & dosage*
Male
Peptide Fragments / blood*
Peptide Fragments / pharmacokinetics
Peptides / chemistry
Rats
Rats, Wistar
Seed Storage Proteins / administration & dosage*
Seed Storage Proteins / blood*
Soybean Proteins / administration & dosage*
Soybean Proteins / blood*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods

Substances

Antigens, Plant
Globulins
Peptide Fragments
Peptides
Seed Storage Proteins
Soybean Proteins
beta-conglycinin protein, Glycine max