Microbiological assessment of aerobically stored horse fillets through predictive microbiology and metabolomic approach

Dimitrios E Pavlidis; Athanasios Mallouchos; George John Nychas

doi:10.1016/j.meatsci.2020.108323

Microbiological assessment of aerobically stored horse fillets through predictive microbiology and metabolomic approach

Meat Sci. 2021 Feb:172:108323. doi: 10.1016/j.meatsci.2020.108323. Epub 2020 Sep 28.

Authors

Dimitrios E Pavlidis¹, Athanasios Mallouchos², George John Nychas³

Affiliations

¹ Laboratory of Microbiology and Biotechnology of Foods, Greece.
² Laboratory of Food Chemistry, Department of Food Science and Human Nutrition, School of Food and Nutritional Sciences, Agricultural University of Athens, IeraOdos 75, Athens 11855, Greece.
³ Laboratory of Microbiology and Biotechnology of Foods, Greece. Electronic address: gjn@aua.gr.

PMID: 33032069
DOI: 10.1016/j.meatsci.2020.108323

Abstract

The aim of this work was to investigate the microbial association of horse fillets during aerobic storage at isothermal conditions (0-15 °C). Samples were analyzed microbiologically, and in parallel the metabolic profile of the samples was quantified by HS-SPME/GC-MS and HPLC-PDA-RI. Considering HPLC results, the concentration of propionic, formic, lactic and succinic acids decreased during aerobic storage of horse fillets, contrary to acetic, citric, butyric and isobutyric acids, which increased. As far as the volatilome formation during aerobic storage is concerned, pentanal, hexanal, octanal, nonanal, decanal, were correlated with fresh samples, while diacetyl, acetoin, 2-heptanone, 2-octanone, hexanoic acid, 3-methyl-butanol, 2-methy-butanol and 3-methyl-butanal detected in spoiled ones. Herein, a support vector machine regression model using data from 0, 5 and 15 °C predicted the responses of the dataset at 10 °C with a correlation coefficient 0.915 and 0.910 for training and testing, respectively.

Keywords: Horsemeat spoilage; Machine learning; Metabolomics; Microbial association; Predictive microbiology; Shelf-life.

MeSH terms

Animals
Food Microbiology*
Food Storage / methods*
Horses
Metabolome
Microbiota
Red Meat / analysis
Red Meat / microbiology*
Volatile Organic Compounds / analysis

Substances

Volatile Organic Compounds