Application of an inhibitor-based probe to reveal the distribution of membrane PSMA in dSTORM imaging

Yingying Jing; Mingjun Cai; Lulu Zhou; Junguang Jiang; Jing Gao; Hongda Wang

doi:10.1039/d0cc04889e

Application of an inhibitor-based probe to reveal the distribution of membrane PSMA in dSTORM imaging

Chem Commun (Camb). 2020 Nov 7;56(86):13241-13244. doi: 10.1039/d0cc04889e. Epub 2020 Oct 8.

Authors

Yingying Jing¹, Mingjun Cai, Lulu Zhou, Junguang Jiang, Jing Gao, Hongda Wang

Affiliation

¹ Research Center of Biomembranomics, State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China. gaojing@ciac.ac.cn hdwang@ciac.ac.cn.

PMID: 33030161
DOI: 10.1039/d0cc04889e

Abstract

Relying on an inhibitor-based probe, we reveal the clustered distribution of membrane PSMA by dSTORM imaging and uncover its potential interaction with folate receptor. This inhibitor-based strategy realizes more accurate labeling than antibody labeling, which would make it a powerful tool in the field of dSTORM imaging.

MeSH terms

Antigens, Surface / analysis*
Cell Membrane / chemistry
Fluorescent Dyes / chemistry*
Glutamate Carboxypeptidase II / analysis*
Humans
Male
Molecular Structure
Optical Imaging*
Prostatic Neoplasms / diagnostic imaging*

Substances

Antigens, Surface
Fluorescent Dyes
FOLH1 protein, human
Glutamate Carboxypeptidase II