Central and peripheral methylamine-induced hypophagia is mediated via nitric oxide and TAAR1 in neonatal layer-type chicken

Abstract

The aim of the current study was to determine effects of intracerebroventricular (ICV) and intraperitoneal (i.p.) administration of Methylamine (MET) and possible interactions with nitric oxide (NO) and TAAR₁ pathways in 24-h fasted (FD₂₄) and ad libitum layer-type chicken. In experiment 1, FD₂₄ chicken ICV injected with MET (15, 30, 45, 60 and 75 μg). In experiment 2, ICV injection of MET (15, 30, 45, 60 and 75 μg) was injected in the ad libitum birds. Experiments 3-4 were similar to experiments 1-2, except chicken i.p. injected with MET (15, 30, 45, 60 and 75 mg/kg). In experiment 5, FD₂₄ birds ICV injected with l-NAME (NO synthesis inhibitor, 100 nmol), MET (75 μg) and co-injection of l-NAME + MET. Experiment 6 was similar to experiment 5, except, ad libitum birds received injections. In experiment 7, FD₂₄ chicken i.p. injected with l-NAME (100 mg/kg), MET (75 mg/kg) and co-injection of l-NAME + MET. In experiment 8, FD₂₄ birds ICV injected with RO5256390 (selective TAAR₁ agonist, 10, 20 and 40 μg). In experiment 9, ad libitum birds ICV injected with RO5256390 (10, 20 and 40 μg). In experiment 10, FD₂₄ birds ICV injected with RO5256390 (10 μg), MET (75 μg) and their co-injection. Experiment 11 was similar to experiment 10, except, ad libitum birds received ICV injections. In experiment 12, FD₂₄ chicken i.p. injected with RO5256390 (2.5, 5 and 10 mg/kg). In experiment 13, FD₂₄ chicken i.p. injected with RO5256390 (2.5 mg/kg), MET (75 mg/kg) and RO5256390 + MET. Then cumulative food intake was determined until 120 min after injection. According to the results, ICV injection of MET decreased food intake in FD₂₄ and ad libitum chicken (P < 0.05). MET (i.p.) diminished food consumption in fasted (P < 0.05) but not in ad libitum chicken (P> 0.05). Co-injection of the l-NAME + MET significantly decreased MET-induced hypophagia in FD₂₄ and ad libitum chicken (P < 0.05). MET-induced hypophagia (i.p.) weakened by l-NAME in FD₂₄ chicken (P < 0.05). RO5256390 decreased food intake in FD₂₄ and ad libitum chicken (P < 0.05). Co-injection of RO5256390 + MET increased MET-induced hypophagia in FD₂₄ and ad libitum chicken (P < 0.05). RO5256390 decreased food intake in FD₂₄ chicken (P < 0.05). Co-injection of the RO5256390 + MET amplified MET-induced hypophagia in FD₂₄ chicken (P < 0.05). Based on the findings, MET-induced hypophagia is mediated via NO and TAAR₁ pathways on food intake in layer chicken.

Keywords: Central and peripheral; Food intake; L-NAME; Layer-type chicken; Methylamine; TAAR(1).