The milestone of genetic screening: Mammalian haploid cells

Shengyi Sun; Yiding Zhao; Ling Shuai

doi:10.1016/j.csbj.2020.09.006

The milestone of genetic screening: Mammalian haploid cells

Comput Struct Biotechnol J. 2020 Sep 12:18:2471-2479. doi: 10.1016/j.csbj.2020.09.006. eCollection 2020.

Authors

Shengyi Sun¹, Yiding Zhao¹, Ling Shuai^{1

2

3}

Affiliations

¹ State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin 300350, China.
² Tate Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
³ Tianjin Central Hospital of Gynecology Obstetrics / Tianjin Key Laboratory of Human Development and Reproductive Regulation, Tianjin 300052, China.

Abstract

Mammalian haploid cells provide insights into multiple genetics approaches as have been proved by advances in homozygous phenotypes and function as gametes. Recent achievements make ploidy of mammalian haploid cells stable and improve the developmental efficiency of embryos derived from mammalian haploid cells intracytoplasmic microinjection, which promise great potentials for using mammalian haploid cells in forward and reverse genetic screening. In this review, we introduce breakthroughs of mammalian haploid cells involving in mechanisms of self-diploidization, forward genetics for various targeting genes and imprinted genes related development.

Keywords: Genetic screening; Imprinted genes; Mammalian haploid cells; Self-diploidization.

Publication types

Review