Antioxidant and ntiinflammatory efficacy of curcumin on lung tissue in rats with sepsis

J Tradit Chin Med. 2020 Oct;40(5):820-826. doi: 10.19852/j.cnki.jtcm.2020.05.012.

Abstract

Objective: To detect antioxidant and antiinflammatory efficacy of Curcumin (Cur) on lung tissue in rats with sepsis.

Methods: Totally 32 rats were divided into 4 groups; the rats in Group 1 (control group) had abdominal incision under sterile conditions following anesthesia and the abdomen was sutured. Abdominal incision was performed in the rats in Group 2 (Cur group) under sterile conditions following anesthesia and the abdomen was closed. Cur was given to this group after dissolving within dimethylsulphoxide as 100 mg/kg through oral gavage and started for 3 d before surgical procedure. Group 3 (CLP group) had caecal ligation and punction (CLP) under sterile conditions to create sepsis following anesthesia and the abdomen was sutured. CLP was performed in the rats in Group 4 (CLP + Cur group) under sterile conditions following anesthesia to create a sepsis model and the abdomen was closed. Cur was also given to this group after dissolving within dimethylsulphoxide as 100 mg/kg through oral gavage and started for 3 d before surgical procedure. All the rats were sacrificed through blood aspiration from the heart under sterile conditions following anesthesia and lung tissues were removed after 24 h following the surgical procedures. The tissue samples were homogenizated for biochemical analyses; and malondialdehyde (MDA), nitric okxide (NO), myeloperoxidase (MPO), superoxidedysmutase (SOD) nd catalase (CAT) were analyzed through spectrophotometric method, immunhistochemical iNOS staining was performed to assess the inflammation; and histopathological differences between the groups were evaluated.

Results: A statistically significant decrease was detected in the CLP + Cur group when compared with the CLP group of which Cur was not given in terms of MDA, MPO and NO levels (P < 0.05) whereas a statistically significant elevation was fpund in the CLP + Cur group when compared with the CLP group in terms of SOD and CAT levels (P < 0.05).

Conclusion: The study outcomes revealed that supplementation of Cur presents an antioxidant effect by reducing the free radical level and increasing the antioxidant enzyme levels; and an antiinflammatory effect by reducing iNOS level.

Keywords: Anti-inflammatory agent; Antioxidant; Curcumin; Nitric Oxide Synthase Type Ⅱ; Sepsi.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / administration & dosage*
  • Antioxidants / administration & dosage*
  • Curcumin / administration & dosage*
  • Humans
  • Lung / drug effects*
  • Lung / immunology
  • Male
  • Malondialdehyde / immunology
  • Nitric Oxide / immunology
  • Oxidative Stress / drug effects
  • Peroxidase / immunology
  • Rats
  • Rats, Wistar
  • Sepsis / drug therapy*
  • Sepsis / genetics
  • Sepsis / immunology
  • Superoxide Dismutase / genetics
  • Superoxide Dismutase / immunology
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / immunology

Substances

  • Anti-Inflammatory Agents
  • Antioxidants
  • Tumor Necrosis Factor-alpha
  • Nitric Oxide
  • Malondialdehyde
  • Peroxidase
  • Superoxide Dismutase
  • Curcumin