Contamination inside CT gantry in the SARS-CoV-2 era

Eur Radiol Exp. 2020 Oct 1;4(1):55. doi: 10.1186/s41747-020-00182-1.

Abstract

We investigated whether the internal gantry components of our computed tomography (CT) scanner contain severe acute respiratory syndrome 2 (SARS-CoV-2) ribonucleic acid (RNA), bacterial or fungal agents. From 1 to 27 March 2020, we performed 180 examinations of patients with confirmed SARS-CoV-2 infection using a dedicated CT scanner. On 27 March 2020, this CT gantry was opened and sampled in each of the following components: (a) gantry case; (b) inward airflow filter; (c) gantry motor; (d) x-ray tube; (e) outflow fan; (f) fan grid; (g) detectors; and (h) x-ray tube filter. To detect SARS-CoV-2 RNA, samples were analysed using reverse transcriptase-polymerase chain reaction (RT-PCR). To detect bacterial or fungal agents, samples have been collected using "replicate organism detection and counting" contact plates of 24 cm2, containing tryptic soy agar, and subsequently cultured. RT-PCR detected SARS-CoV-2 RNA in the inward airflow filter sample. RT-PCR of remaining gantry samples did not reveal the presence of SARS-CoV-2 RNA. Neither bacterial nor fungal agents grew in the agar-based growth medium after the incubation period. Our data showed that SARS-Cov-2 RNA can be found inside the CT gantry only in the inward airflow filter. All remaining CT gantry components were devoid of SARS-CoV-2 RNA.

Keywords: COVID-19; Patient safety; Safety management; Severe acute respiratory syndrome coronavirus 2; Tomography scanners (x-ray computed).

MeSH terms

  • Betacoronavirus / isolation & purification*
  • COVID-19
  • Coronavirus Infections / virology*
  • Equipment Contamination*
  • Humans
  • Pandemics
  • Pneumonia, Viral / virology*
  • RNA, Viral / isolation & purification
  • Real-Time Polymerase Chain Reaction
  • SARS-CoV-2
  • Tomography Scanners, X-Ray Computed / virology*
  • Tomography, X-Ray Computed / instrumentation*

Substances

  • RNA, Viral