To analyze physicochemical such as surface structures, the crystallinity, chemical composition, calcium phosphate dissolution and osteogenic properties of tooth derived bone substitute (TDBS) processed chair-side and other grafting materials. The number of anaerobic and facultative anaerobic bacteria in the supernatant of processed TDBS was determined. Human osteoblasts were co-cultured with TDBS or allograft in transwell system to examine cell migration. BMP2 released from TDBS was measured by ELISA. TDBS had high crystallinity similar to BoneCeramic while it had a broad pattern to ramus bone, OraGRAFT, and Bio-Oss. TDBS contained carbon, calcium, oxygen, phosphate, sodium and magnesium elements like others. Calcium/phosphorus dissolution of TDBS show closely related to those of mandibular ramus bone and OraGRAFT. In addition, microbial decontamination of TDBS by the chemical processing revealed a hundred percent efficacy. The osteoconductive and osteoinductive properties demonstrated in the TDBS processed chairside suggested the potential of an alternative for bone grafting material.
Keywords: Demineralized dentin matrix; Dental material; Dentin grinder; Ridge augmentation; XRD analysis.