Data for microbe resistant engineered recombinant spider silk protein based 2D and 3D materials

Sushma Kumari; Gregor Lang; Elise DeSimone; Christian Spengler; Vanessa T Trossmann; Susanne Lücker; Martina Hudel; Karin Jacobs; Norbert Krämer; Thomas Scheibel

doi:10.1016/j.dib.2020.106305

Data for microbe resistant engineered recombinant spider silk protein based 2D and 3D materials

Data Brief. 2020 Sep 11:32:106305. doi: 10.1016/j.dib.2020.106305. eCollection 2020 Oct.

Authors

Sushma Kumari¹, Gregor Lang^{1

2}, Elise DeSimone¹, Christian Spengler³, Vanessa T Trossmann¹, Susanne Lücker⁴, Martina Hudel⁵, Karin Jacobs³, Norbert Krämer⁴, Thomas Scheibel^{1

6

7

8

9}

Affiliations

¹ Department of Biomaterials, Faculty of Engineering Science, University of Bayreuth, Prof.-Rüdiger-Bormann-Str. 1, 95447 Bayreuth, Germany.
² Biopolymer Processing Group, Faculty of Engineering Science, University of Bayreuth, Ludwig-Thoma-Str. 36A, 95447 Bayreuth, Germany.
³ Department of Experimental Physics and Center for Biophysics, Saarland University, 66123 Saarbrücken, Germany.
⁴ Medical Center for Dentistry, Department of Paediatric Dentistry, Medical Center Gießen and Marburg, Justus-Liebig University Gießen, Schlagenzahl 14, 35392 Gießen, Germany.
⁵ Institute of Medical Microbiology, Justus-Liebig University Gießen, Schubertstraße 81, 35392 Gießen, Germany.
⁶ Bayreuth Center for Material Science and Engineering (BayMAT), Germany.
⁷ Bavarian Polymer Institute (BPI), Germany.
⁸ Bayreuth Center for Colloids and Interfaces (BZKG), Germany.
⁹ Bayreuth Center for Molecular Biosciences (BZMB), University of Bayreuth, Universitätsstraße 30, 95447 Bayreuth, Germany.

Abstract

Data presented in this article describe bacterial and fungal repellent properties of 2D-films and 3D-hydrogels made of different recombinantly produced spider silk proteins based on consensus sequences of Araneus diadematus dragline silk proteins (fibroin 3 and 4). Here, the attachment, growth, and microbial colonization of Streptococcus mutans (S. mutans) as well as Candida albicans (C. albicans) on plane and micro-patterned films were visualized by scanning electron microscopy (SEM). Also, microbial viability data are provided of Escherichia coli (E. coli) and Pichia pastoris (P. pastoris) on hydrogels made of eADF4(C16) and eADF4(C16)-RGD, quantified using the Alamar blue assay. Experimental results, design of a post-operative contamination model of microbes with mammalian cells, and methods in the data article refer to the research paper "Engineered spider silk-based 2D and 3D materials prevent microbial infestation" published recently [1].

Keywords: Biofilm; Bioselective surface; Engineered spider silk proteins; Hydrogels; Microbe adhesion; Patterned films.