MiR-4334-5p Facilitates Foot and Mouth Disease Virus Propagation by Suppressing Interferon Pathways via Direct Targeting ID1

Genes (Basel). 2020 Sep 27;11(10):1136. doi: 10.3390/genes11101136.

Abstract

Emerging evidence indicates that the host microRNAs (miRNAs) are important intracellular regulators and play pivotal roles in intricate host-pathogen interaction networks. In our previous studies, ssc-microRNA-4334-5p (miR-4334-5p) was identified as a differentially expressed miRNA in microarray-based miRNAs profiling experiment, but whether miR-4334-5p regulates foot and mouth disease virus (FMDV) propagation is less understood. Here, we demonstrated that miR-4334-5p expression level was up-regulated shortly after FMDV infection, transfection of miR-4334-5p mimics promoted, while inhibitor transfection suppressed FMDV replication correspondingly. Further bioinformatic analysis and experimental study suggested ID1 was the direct target of miR-4334-5p, suppressing FMDV replication by regulating interferon (IFN) pathways. These findings shed light on microRNAs-ID1-interferon axis in regulating FMDV replication.

Keywords: FMDV; ID1; interferon; miR-4334-5p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Cricetinae
  • Foot-and-Mouth Disease / genetics
  • Foot-and-Mouth Disease / metabolism
  • Foot-and-Mouth Disease / virology*
  • Foot-and-Mouth Disease Virus / pathogenicity*
  • Host-Pathogen Interactions*
  • Inhibitor of Differentiation Protein 1 / antagonists & inhibitors*
  • Interferon Type I / antagonists & inhibitors*
  • Kidney / metabolism
  • Kidney / virology*
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Signal Transduction
  • Swine

Substances

  • Inhibitor of Differentiation Protein 1
  • Interferon Type I
  • MicroRNAs