Neutralizing antibody response elicited by SARS-CoV-2 receptor-binding domain

Ping-Han Huang; Hsiao-Han Tsai; Bo-Hung Liao; Yi-Ling Lin; Jia-Tsrong Jan; Mi-Hua Tao; Yu-Chi Chou; Che-Ming Jack Hu; Hui-Wen Chen

doi:10.1080/21645515.2020.1814098

Neutralizing antibody response elicited by SARS-CoV-2 receptor-binding domain

Hum Vaccin Immunother. 2021 Mar 4;17(3):654-655. doi: 10.1080/21645515.2020.1814098. Epub 2020 Sep 29.

Authors

Ping-Han Huang¹, Hsiao-Han Tsai^{2

3}, Bo-Hung Liao², Yi-Ling Lin², Jia-Tsrong Jan⁴, Mi-Hua Tao², Yu-Chi Chou⁵, Che-Ming Jack Hu², Hui-Wen Chen¹

Affiliations

¹ Department of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
² Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
³ Taiwan International Graduate Program in Molecular Medicine, National Yang-Ming University and Academia Sinica, Taipei, Taiwan.
⁴ Genomics Research Center, Academia Sinica, Taipei, Taiwan.
⁵ RNAi Core Facility, Academia Sinica, Taipei, Taiwan.

Abstract

A safe and effective vaccine candidate is urgently needed for the ongoing COVID-19 pandemic, caused by SARS-CoV-2. Here we report that recombinant SARS-CoV-2 RBD protein immunization in mice is able to elicit a strong antibody response and potent neutralizing capability as measured using live or pseudotyped SARS-CoV-2 neutralization assays.

Keywords: SARS-CoV-2; neutralizing antibodies; receptor-binding domain; vaccine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Neutralizing / immunology*
Antibodies, Viral / immunology*
COVID-19 / immunology*
Cell Line
HEK293 Cells
Humans
Mice
Pandemics / prevention & control
Protein Binding / immunology*
Protein Domains / immunology*
Recombinant Proteins / immunology
SARS-CoV-2 / immunology*
Spike Glycoprotein, Coronavirus / immunology

Substances

Antibodies, Neutralizing
Antibodies, Viral
Recombinant Proteins
Spike Glycoprotein, Coronavirus

Grants and funding

This study was supported by the Ministry of Science and Technology (MOST-109-3114-Y-001-001 and MOST-109-2327-B-002-009), National Taiwan University and Academia Sinica, Taiwan.