Identification of transcriptome differences in goat ovaries at the follicular phase and the luteal phase using an RNA-Seq method

Yong Liu; Xiaoqing Wu; Juan Xie; Wenying Wang; Jing Xin; Feng Kong; Qiaoqin Wu; Yinghui Ling; Xinyan Cao; Qingmei Li; Shengnan Zhang; Ling Ding; Xudong Wang; Fengrui Wu; Di Zhang; Rong Wang; Wei Cui; Xiaorong Zhang; Wenyong Li

doi:10.1016/j.theriogenology.2020.06.045

Identification of transcriptome differences in goat ovaries at the follicular phase and the luteal phase using an RNA-Seq method

Theriogenology. 2020 Dec:158:239-249. doi: 10.1016/j.theriogenology.2020.06.045. Epub 2020 Aug 31.

Authors

Yong Liu¹, Xiaoqing Wu², Juan Xie², Wenying Wang², Jing Xin², Feng Kong², Qiaoqin Wu², Yinghui Ling³, Xinyan Cao⁴, Qingmei Li², Shengnan Zhang², Ling Ding², Xudong Wang², Fengrui Wu², Di Zhang², Rong Wang², Wei Cui⁵, Xiaorong Zhang³, Wenyong Li⁶

Affiliations

¹ Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, Fuyang Normal University, Fuyang City, Anhui Province, 236041, China. Electronic address: liuyong@fynu.edu.cn.
² Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, Fuyang Normal University, Fuyang City, Anhui Province, 236041, China.
³ Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui, 230036, China.
⁴ Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No.4899 Juye Street, Jingyue District, Changchun, 130112, China.
⁵ Department of Veterinary and Animal Sciences, Animal Models Core Facility, Institute for Applied Life Sciences (IALS), University of Massachusetts, Amherst, MA, 01002, USA.
⁶ Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, Fuyang Normal University, Fuyang City, Anhui Province, 236041, China. Electronic address: liwenyong@fynu.edu.cn.

PMID: 32987289
DOI: 10.1016/j.theriogenology.2020.06.045

Abstract

The ovaries, the main female reproductive organs, directly mediate ovulation and reproductive hormone secretion. These complex physiological processes are regulated by multiple genes and pathways. However, there is a lack of research on goat ovaries, and the molecular mechanisms underlying the signaling pathways remain unclear. In this study, Illumina HiSeq 4000 sequencing was used to sequence the transcriptomes of goat ovaries. The expression patterns of differentially expressed mRNAs in goat ovaries at both the follicular and luteal phases were determined by bioinformatics analysis. A total of 1,122, 014, 112 clean reads were obtained, and 3770 differentially expressed mRNAs were identified for further analysis. There were 1727 and 2043 upregulated mRNAs in the luteal phase and follicular phase, respectively. According to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, some mRNAs that were highly expressed in ovaries during the luteal phase, such as HSD17B7, 3BHSD, and SRD5A2, may be related to the synthesis of progesterone. In addition, some mRNAs that were highly expressed in ovaries during the follicular phase, such as RPL12, RPS13 and RPL10, are related to the growth and maturation of oocytes. Taken together, the findings of this study provide genome-wide mRNA expression profiles for goat ovaries at the follicular and luteal phases and identify mRNAs associated with goat hormone secretion and follicular development. In addition, this study provides a theoretical basis for further investigation of goat reproductive regulation.

Keywords: Differential gene; Genome-wide; Goat ovary; mRNA.

MeSH terms

Animals
Female
Follicular Phase* / genetics
Goats / genetics
Luteal Phase* / genetics
Ovary
RNA-Seq / veterinary
Transcriptome