The lower regeneration rate of wheat calli is the main factor restricting the development of transgenic wheat plants. Therefore, improving the regeneration rate of wheat callus is a precondition for developing genetic engineering-based wheat breeding approaches. In the present study, we explored the molecular mechanism of wheat regeneration and aimed to establish an efficient system for transgenic wheat. We isolated and identified a regeneration-related gene, TaTCP-1 (KC808517), from wheat cultivar Lunxuan 987. Sequence analysis revealed that the ORF of TaTCP-1 was 1623bp long encoding 540 amino acids. The TaTCP-1 gene was expressed in various wheat tissues. Further, the level of TaTCP-1 expression was higher in calli and increased gradually with increasing callus induction time, reaching a peak on the 11th day after induction. Moreover, the expression level of TaTCP-1 was higher in embryogenic calli than in non-embryonic calli. The TaTCP-1 protein was localized to the nucleus, cytoplasm, and cell membrane. The callus regeneration rate of wheat plants transformed with TaTCP-1-RNAi reduced by 85.09%. In contrast, it increased by 14.43% in plants overexpressing TaTCP-1. In conclusion, our results showed that TaTCP-1 played a vital role in promoting wheat regeneration, and regulated the somatic embryogenesis of wheat. These results may have implications in the genetic engineering of wheat for improved wheat production.
Keywords: TaTCP-1; callus regeneration frequency; gene transformation; somatic embryogenesis; wheat.
Copyright © 2020 Li, Li, Qiao, Li, Guo, Zhang and Min.