Decorated Au NPs on agar modified Fe3O4 NPs: Investigation of its catalytic performance in the degradation of methylene orange, and anti-human breast carcinoma properties

Tao Sun; Jun Gao; Hongyan Shi; Dan Han; Mohammad Mahdi Zangeneh; Na Liu; Hui Liu; Yanhong Guo; Xianqiang Liu

doi:10.1016/j.ijbiomac.2020.09.157

Decorated Au NPs on agar modified Fe₃O₄ NPs: Investigation of its catalytic performance in the degradation of methylene orange, and anti-human breast carcinoma properties

Int J Biol Macromol. 2020 Dec 15;165(Pt A):787-795. doi: 10.1016/j.ijbiomac.2020.09.157. Epub 2020 Sep 24.

Authors

Tao Sun¹, Jun Gao¹, Hongyan Shi², Dan Han³, Mohammad Mahdi Zangeneh⁴, Na Liu¹, Hui Liu¹, Yanhong Guo¹, Xianqiang Liu⁵

Affiliations

¹ Department of Breast and Thyroid Surgery, Jinan Central Hospital Affiliated to Shandong First Medical University, No. 105 Jiefang road, Jinan, Shandong Province 250013, China.
² Department of ENT(ear-nose-throat), Jinan Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong Province 250013, China.
³ Eye, Plastic and Oral Wards, Jinan Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong Province 250013, China.
⁴ Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran; Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran.
⁵ Department of Breast and Thyroid Surgery, Jinan Central Hospital Affiliated to Shandong First Medical University, No. 105 Jiefang road, Jinan, Shandong Province 250013, China. Electronic address: liuxq126@tom.com.

PMID: 32980407
DOI: 10.1016/j.ijbiomac.2020.09.157

Abstract

This work describes an eco-friendly approach for in situ immobilization of Au nanoparticles on the surface of Fe₃O₄ nanoparticles, with help of Agar and ultrasound irradiations, without using any toxic reducing and capping agents. The structure, morphology, and physicochemical properties were characterized by various analytical techniques such as Fourier transformed infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), inductively coupled plasma (ICP) and vibrating sample magnetometer (VSM). The desired catalyst showed great efficiency in the reductive degradation of methylene orange (MO) dye over NaBH₄ at room temperature. The MO was fully reduced in only 70 s and achieved rate constant of 9.6 × 10^-2 s^-1. The catalyst was reused for 10 runs without significant loss in catalytic activity. Cell viability of Fe₃O₄/agar/Au NPs was very low against breast adenocarcinoma (MCF7), breast carcinoma (Hs 578Bst), infiltrating ductal cell carcinoma (Hs 319.T), and metastatic carcinoma (MDA-MB-453) cell lines without any cytotoxicity on the normal cell line. According to the above findings, the Fe₃O₄/agar/Au NPs may be administrated for the treatment of several types of human breast carcinoma in humans.

Keywords: Gold nanoparticles; Human breast carcinoma; Methylene orange.

MeSH terms

Agar* / chemistry
Agar* / pharmacology
Breast Neoplasms / drug therapy*
Breast Neoplasms / metabolism
Breast Neoplasms / pathology
Catalysis
Female
Fluorescent Dyes / chemistry*
Gold* / chemistry
Gold* / pharmacology
Humans
MCF-7 Cells
Magnetite Nanoparticles* / chemistry
Magnetite Nanoparticles* / therapeutic use
Metal Nanoparticles* / chemistry
Metal Nanoparticles* / therapeutic use

Substances

Fluorescent Dyes
Magnetite Nanoparticles
Gold
Agar