Expansion and Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

Roberto Narcisi; Wendy J L M Koevoet; Gergo J V M van Osch

doi:10.1007/978-1-0716-0989-7_2

Expansion and Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

Methods Mol Biol. 2021:2221:15-28. doi: 10.1007/978-1-0716-0989-7_2.

Authors

Roberto Narcisi¹, Wendy J L M Koevoet², Gergo J V M van Osch^{3

2}

Affiliations

¹ Department of Orthopedics, Erasmus MC, University Medical Center, Rotterdam, The Netherlands. n.narcisi@erasmus.nl.
² Department of Otorhinolaryngology, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
³ Department of Orthopedics, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.

PMID: 32979195
DOI: 10.1007/978-1-0716-0989-7_2

Abstract

Human bone marrow-derived mesenchymal stem/stromal cells (BM-MSC) are adult multipotent progenitor cells that can be isolated from bone marrow. BM-MSCs have the ability to be expanded and differentiated into the chondrogenic lineage in vitro. Here we describe a standardized method to expand and chondrogenically differentiate human BM-MSCs, highlighting how to overcome technical challenges and indicating the most common readout parameters to evaluate the chondrogenic differentiation capacity.

Keywords: Cartilage; Chondrogenesis; Mesenchymal stem cells; Regenerative medicine; Tissue engineering.

MeSH terms

Cell Differentiation*
Cells, Cultured
Chondrogenesis
Humans
Mesenchymal Stem Cells / cytology*
Tissue Engineering / methods*