Cultivation of diatom Pinnularia saprophila for lipid production: A comparison of methods for harvesting the lipid from the cells

Abstract

The present study underlines the application of centrifugal force and pulse electric field techniques along with its comparison to resonance energy to harvest lipid from a fixed number of Pinnularia saprophila cells. Sulpho phospho vanillin method for lipid, and analysis of cells via microscopy was done. It was found that a centrifugal force of 11110×g for 15 min allowed ~3.39% lipid to ooze out with 2.5% cell destruction. Alternatively, when same numbers of diatom cells were subjected to pulse electric field at 110 kV/27 mA for 10 µs, maximum lipid production of 2.86% with 21.19% cell death was observed. It was perceived that diatom cells in a micro resonating micro fluidic chamber for 20 min harvested 4.4% of lipid with 11.16% of cell death. However, microfluidic device needs to be scaled up using cheaper material instead of silicon wafer, to be an efficient technique to milk lipid from diatoms.

Keywords: Centrifugal force; Diatoms; Lipid; Pulse electric field; Resonance energy.