Evaluation of Murine Macrophage Cytokine Production After In Vivo Morphine Treatment

Silvia Franchi; Mara Castelli; Sarah Moretti; Alberto Panerai; Paola Sacerdote

doi:10.1007/978-1-0716-0884-5_18

Evaluation of Murine Macrophage Cytokine Production After In Vivo Morphine Treatment

Methods Mol Biol. 2021:2201:199-207. doi: 10.1007/978-1-0716-0884-5_18.

Authors

Silvia Franchi¹, Mara Castelli¹, Sarah Moretti¹, Alberto Panerai¹, Paola Sacerdote²

Affiliations

¹ Department of Pharmacological and Biomolecular Science, University of Milano, Milan, Italy.
² Department of Pharmacological and Biomolecular Science, University of Milano, Milan, Italy. paola.sacerdote@unimi.it.

PMID: 32975801
DOI: 10.1007/978-1-0716-0884-5_18

Abstract

The discovery of opioid receptor expression on immune cells has originated a large research activity on the possible modulation by opioid drugs of immune system responses. In this chapter we describe an easy methodology useful to obtain information about the potential immunomodulatory activity of opioid drugs. An in vivo treatment schedule is used, and macrophages are studied for their ability to release different cytokines.

Keywords: Cytokines; Lipopolysaccharide; Morphine; Murine macrophages; Opioid-induced immunosuppression.

MeSH terms

Analgesics, Opioid / immunology
Analgesics, Opioid / pharmacology
Animals
Cytokines / analysis*
Cytokines / drug effects
Immune System Phenomena
Immunologic Factors / analysis
Immunologic Factors / pharmacology
Immunomodulation / drug effects
Immunomodulation / immunology
Macrophages / drug effects*
Macrophages / metabolism
Mice
Morphine / adverse effects
Morphine / metabolism
Morphine / pharmacology
Primary Cell Culture / methods*
Receptors, Opioid / immunology

Substances

Analgesics, Opioid
Cytokines
Immunologic Factors
Receptors, Opioid
Morphine