Fluorescence Colocalization Analysis of Cellular Distribution of MOR-1

Vittoria Borgonetti; Nicoletta Galeotti

doi:10.1007/978-1-0716-0884-5_3

Fluorescence Colocalization Analysis of Cellular Distribution of MOR-1

Methods Mol Biol. 2021:2201:27-34. doi: 10.1007/978-1-0716-0884-5_3.

Authors

Vittoria Borgonetti¹, Nicoletta Galeotti²

Affiliations

¹ Department of Neuroscience, Psychology, Drug Research and Child Health (Neurofarba), University of Florence, Florence, Italy.
² Department of Neuroscience, Psychology, Drug Research and Child Health (Neurofarba), University of Florence, Florence, Italy. nicoletta.galeotti@unifi.it.

PMID: 32975786
DOI: 10.1007/978-1-0716-0884-5_3

Abstract

The interaction between neurons and glia is pivotal for the development of chronic opioid tolerance. One of the most important mechanisms of cell-to-cell interaction is the Notch signaling pathway. In this chapter we propose a double-immunofluorescence method to observe and quantify the colocalization of Notch-1 and mu-opioid receptor (MOR-1), using both neuronal and astrocyte markers.

Keywords: Colocalization; Immunofluorescence; Morphine; Mu-opioid receptor; Notch signaling; Tolerance.

MeSH terms

Analgesics, Opioid / metabolism
Animals
Drug Tolerance
Fluorescence
Fluorescent Antibody Technique, Direct / methods*
Humans
Neurons / metabolism
Receptor, Notch1 / metabolism*
Receptors, Notch / metabolism
Receptors, Opioid, mu / metabolism*
Signal Transduction / drug effects

Substances

Analgesics, Opioid
OPRM1 protein, human
Receptor, Notch1
Receptors, Notch
Receptors, Opioid, mu