Effect of various bacteriological media on the plaque morphology of Staphylococcus and Vibrio phages

Nachimuthu Ramesh; Loganathan Archana; Madhav Madurantakam Royam; Prasanth Manohar; Kandasamy Eniyan

doi:10.1099/acmi.0.000036

Effect of various bacteriological media on the plaque morphology of Staphylococcus and Vibrio phages

Access Microbiol. 2019 Jun 17;1(4):e000036. doi: 10.1099/acmi.0.000036. eCollection 2019.

Authors

Nachimuthu Ramesh¹, Loganathan Archana¹, Madhav Madurantakam Royam¹, Prasanth Manohar¹, Kandasamy Eniyan¹

Affiliation

¹ Antibiotic Resistance and Phage Therapy Laboratory, School of Bio Science and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India.

Abstract

The influence of media composition on the life cycle of bacteriophages to exhibit diverse plaque morphology on various bacteriological media was investigated by a double agar overlay method. Both Staphylococcus aureus phage and Vibrio parahaemolyticus phage showed altered plaque morphology from small to large and from clear to turbid, in different culture media used for the double agar overlay method.

Keywords: bacteriophage; lytic-lysogenic shift; phage therapy; plaque assay; plaque morphology.