High-quality draft genome sequences of Pseudomonas monteilii DSM 14164T, Pseudomonas mosselii DSM 17497T, Pseudomonas plecoglossicida DSM 15088T, Pseudomonas taiwanensis DSM 21245T and Pseudomonas vranovensis DSM 16006T: taxonomic considerations

Arantxa Peña; Antonio Busquets; Margarita Gomila; Magdalena Mulet; Rosa M Gomila; Elena Garcia-Valdes; T B K Reddy; Marcel Huntemann; Neha Varghese; Natalia Ivanova; I-Min Chen; Markus Göker; Tanja Woyke; Hans-Peter Klenk; Nikos Kyrpides; Jorge Lalucat

doi:10.1099/acmi.0.000067

High-quality draft genome sequences of Pseudomonas monteilii DSM 14164^T, Pseudomonas mosselii DSM 17497^T, Pseudomonas plecoglossicida DSM 15088^T, Pseudomonas taiwanensis DSM 21245^T and Pseudomonas vranovensis DSM 16006^T: taxonomic considerations

Access Microbiol. 2019 Oct 29;1(10):e000067. doi: 10.1099/acmi.0.000067. eCollection 2019.

Authors

Arantxa Peña¹, Antonio Busquets¹, Margarita Gomila¹, Magdalena Mulet¹, Rosa M Gomila², Elena Garcia-Valdes^{1

3}, T B K Reddy⁴, Marcel Huntemann⁴, Neha Varghese⁴, Natalia Ivanova⁴, I-Min Chen⁴, Markus Göker⁵, Tanja Woyke⁴, Hans-Peter Klenk⁶, Nikos Kyrpides⁴, Jorge Lalucat^{1

3}

Affiliations

¹ Department of Biology-Microbiology, Universitat de les Illes Balears, Palma de, Mallorca, Spain.
² Serveis Cientifico-Tècnics, Universitat de les Illes Balears, Palma de Mallorca, Spain.
³ Institut Mediterrani d'Estudis Avançats (IMEDEA, CSIC-UIB), Palma de Mallorca, Spain.
⁴ DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA.
⁵ Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, Germany.
⁶ School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.

Abstract

Pseudomonas is the bacterial genus of Gram-negative bacteria with the highest number of recognized species. It is divided phylogenetically into three lineages and at least 11 groups of species. The Pseudomonas putida group of species is one of the most versatile and best studied. It comprises 15 species with validly published names. As a part of the Genomic Encyclopedia of Bacteria and Archaea (GEBA) project, we present the genome sequences of the type strains of five species included in this group: Pseudomonas monteilii (DSM 14164^T), Pseudomonas mosselii (DSM 17497^T), Pseudomonas plecoglossicida (DSM 15088^T), Pseudomonas taiwanensis (DSM 21245^T) and Pseudomonas vranovensis (DSM 16006^T). These strains represent species of environmental and also of clinical interest due to their pathogenic properties against humans and animals. Some strains of these species promote plant growth or act as plant pathogens. Their genome sizes are among the largest in the group, ranging from 5.3 to 6.3 Mbp. In addition, the genome sequences of the type strains in the Pseudomonas taxonomy were analysed via genome-wide taxonomic comparisons of ANIb, gANI and GGDC values among 130 Pseudomonas strains classified within the group. The results demonstrate that at least 36 genomic species can be delineated within the P. putida phylogenetic group of species.

Keywords: Genomic Encyclopedia of Bacteria and Archaea (GEBA); P. monteilii; P. mosselii; P. plecoglossicida; P. taiwanensis; P. vranovensis; Thousand Microbial Genomes Project (KMG I); taxonomy; type strains.