pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110

Lena Schaffert; Lucas Jacob; Susanne Schneiker-Bekel; Marcus Persicke; Camilla März; Christian Rückert; Alfred Pühler; Jörn Kalinowski

doi:10.1128/MRA.00596-20

pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110

Microbiol Resour Announc. 2020 Sep 24;9(39):e00596-20. doi: 10.1128/MRA.00596-20.

Authors

Lena Schaffert¹, Lucas Jacob¹, Susanne Schneiker-Bekel^{1

2}, Marcus Persicke¹, Camilla März¹, Christian Rückert¹, Alfred Pühler², Jörn Kalinowski³

Affiliations

¹ Microbial Genomics and Biotechnology, Center for Biotechnology, Bielefeld University, Bielefeld, Germany.
² Senior Research Group in Genome Research of Industrial Microorganisms, Center for Biotechnology, Bielefeld University, Bielefeld, Germany.
³ Microbial Genomics and Biotechnology, Center for Biotechnology, Bielefeld University, Bielefeld, Germany joern@CeBiTec.Uni-Bielefeld.DE.

Abstract

The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other Actinomycetales by exchanging the promoter.