Antimicrobial susceptibility and genetic features of a heterogeneous vancomycin intermediate-resistant Staphylococcus aureus strain

Liting Wan; Congxiu Ye; Bing Li; Thanapop Soteyome; Xuerui Bao; Zerong Lu; Wenyi Xu; Yuzhu Mao; Lin Li; Dingqiang Chen; Ling Yang; Zhenbo Xu; Janette Harro

doi:10.1016/j.meegid.2020.104565

Antimicrobial susceptibility and genetic features of a heterogeneous vancomycin intermediate-resistant Staphylococcus aureus strain

Infect Genet Evol. 2020 Nov:85:104565. doi: 10.1016/j.meegid.2020.104565. Epub 2020 Sep 21.

Authors

Liting Wan¹, Congxiu Ye², Bing Li³, Thanapop Soteyome⁴, Xuerui Bao³, Zerong Lu³, Wenyi Xu³, Yuzhu Mao³, Lin Li³, Dingqiang Chen⁵, Ling Yang⁶, Zhenbo Xu⁷, Janette Harro⁸

Affiliations

¹ School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510640, China; Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, MD 21201, USA.
² Department of Dermato-Venereology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510640, China.
³ School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510640, China.
⁴ Home Economics Technology, Rajamangala University of Technology Phra Nakhon, Bangkok, Thailand.
⁵ Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
⁶ Department of Laboratory Medicine, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China. Electronic address: jykresearch@126.com.
⁷ School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510640, China; Home Economics Technology, Rajamangala University of Technology Phra Nakhon, Bangkok, Thailand; Department of Clinical Pharmacy and Translational Science, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN 38103, USA; Research Center of Translational Medicine, Second Affiliated Hospital of Shantou University Medical College, Shantou 515041, China; National Institute of Fundamental Studies, Hantana road, Kandy, Sri Lanka. Electronic address: zhenbo.xu@hotmail.com.
⁸ Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, MD 21201, USA.

PMID: 32971249
DOI: 10.1016/j.meegid.2020.104565

Abstract

This study aimed to characterize the antimicrobial susceptibility and genetic features of a heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) strain Guangzhou-SauVS2 recovered from a female patient in Guangzhou, representative of southern China. The genome of Guangzhou-SauVS2 was sequenced using Illumina HiSeq 2500 platform and assembled de novo using Velvet v1.2.08. Annotations and bioinformatics analysis were further performed. Results showed that Guangzhou-SauVS2 was susceptible and resistant to 7 and 11 antibiotic drugs, respectively, and exhibited hVISA with a minimum inhibitory concentration of vancomycin as 4 μg/mL. Its genome is 2,883,941 bp in length and contains 2934 predicted genes with an average G + C content of 32.9%. Besides, a total of 38 virulence factors and 4 antibiotic-resistant genes were identified. These results can be employed to further study the pathogenic and antimicrobial mechanisms of hVISA.

Keywords: Antimicrobial resistance; Genetic characteristics; Heterogeneous vancomycin-intermediated Staphylococcus aureus; Virulence factors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
China
Female
Genome, Bacterial
Genomics / methods
Humans
Microbial Sensitivity Tests
Staphylococcal Infections / drug therapy
Staphylococcal Infections / microbiology*
Staphylococcus aureus / drug effects*
Staphylococcus aureus / genetics*
Vancomycin / pharmacology*
Vancomycin Resistance*
Virulence Factors / genetics

Substances

Anti-Bacterial Agents
Virulence Factors
Vancomycin