Determination of genetic variation within the DYRK2 gene and its associations with milk traits in cattle

Cui Mao; Xing Ju; Haijian Cheng; Xixia Huang; Fugui Jiang; Yuni Yao; Xianyong Lan; Enliang Song

doi:10.5194/aab-63-315-2020

Determination of genetic variation within the DYRK2 gene and its associations with milk traits in cattle

Arch Anim Breed. 2020 Sep 9;63(2):315-323. doi: 10.5194/aab-63-315-2020. eCollection 2020.

Authors

Cui Mao^#¹, Xing Ju^#^{2

3}, Haijian Cheng¹, Xixia Huang², Fugui Jiang¹, Yuni Yao¹, Xianyong Lan³, Enliang Song^{1

4}

Affiliations

¹ Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250000, China.
² College of Animal Science and Technology, Xinjiang Agricultural University, Urumqi, Xinjiang, 830000, China.
³ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
⁴ College of Life Sciences, Shandong Normal University, Jinan, Shandong, 250000, China.

^# Contributed equally.

Abstract

To speed up the progress of marker-assisted selection (MAS) in cattle breeding, the dual-specificity tyrosine phosphorylation-regulated kinase 2 (DYRK2), cadherin 2 (CDH2), and kinesin family member 1A (KIF1A) genes were chosen based on our pervious genome-wide association study (GWAS) analysis results. DYRK2 is a kinase that may participate in cell growth and/or development; it shows phosphorylation activity toward serine, threonine, and tyrosine fragments of proteins, and it is different from other protein kinases. The CDH2 gene encodes a classic cadherin, which is a member of the cadherin superfamily. The protein encoded by KIF1A is a member of the kinesin family and plays a role in the transportation of membrane organelles along axon microtubules. We detected insertion/deletion (InDel) variation in these three candidate genes in 438 individual cattle (Xinjiang Brown cattle and Wagyu $\times$ Luxi crossbreed cattle). Only DYRK2-P3-11 bp was polymorphic and genotyped. The polymorphism information content of DYRK2-P3-11 bp was 0.336. Correlation analyses showed that InDel polymorphism was significantly associated with six different milk traits. These findings may aid future analyses of InDel genotypes in cattle breeds, and speed up the progress of MAS in cattle breeding.