High-intensity ultrasound irradiation promotes the release of extracellular vesicles from C2C12 myotubes

Noriaki Maeshige; P Kent Langston; Zhi-Min Yuan; Hiroyo Kondo; Hidemi Fujino

doi:10.1016/j.ultras.2020.106243

High-intensity ultrasound irradiation promotes the release of extracellular vesicles from C2C12 myotubes

Ultrasonics. 2021 Feb:110:106243. doi: 10.1016/j.ultras.2020.106243. Epub 2020 Sep 5.

Authors

Noriaki Maeshige¹, P Kent Langston², Zhi-Min Yuan³, Hiroyo Kondo⁴, Hidemi Fujino⁵

Affiliations

¹ Department of Genetics & Complex Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA; Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, Japan. Electronic address: nmaeshige@pearl.kobe-u.ac.jp.
² Department of Genetics & Complex Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA; Department of Immunology, Harvard Medical School and Evergrande Center for Immunologic Diseases, Harvard Medical School, Boston, MA, USA.
³ Department of Genetics & Complex Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
⁴ Department of Food Sciences and Nutrition, Nagoya Women's University, Nagoya, Japan.
⁵ Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, Japan.

PMID: 32961400
DOI: 10.1016/j.ultras.2020.106243

Abstract

Skeletal muscle is an important secretory organ in mammals, producing myriad chemical mediators ("myokines") with distinct biological action in different tissues, including anti-inflammatory activity. Extracellular vesicles (EVs) have recently been identified as a mode of myokine transport from muscle, facilitating such anti-inflammatory activity. In this report, we have demonstrated that high-intensity ultrasound (US) strongly induces EV secretion from cultured myotubes without a reduction in cell viability. High-intensity US of 3.0 W/cm² with 20% duty cycle increased the number of EVs by 2-fold compared to control at 6 h. This effect was specific to EVs in the 100-150 nm size range. Thus, high-intensity US is a novel modality for inducing myocellular EV release and may hold therapeutic value.

Keywords: Extracellular vesicles; High-intensity ultrasound; Myotubes.

MeSH terms

Animals
Cells, Cultured
Extracellular Vesicles / metabolism*
Extracorporeal Shockwave Therapy / methods*
Mice
Muscle Fibers, Skeletal / metabolism*