Bioreactor-Based Antigen Production Process Using the Baculovirus Expression Vector System

Methods Mol Biol. 2021:2183:95-118. doi: 10.1007/978-1-0716-0795-4_8.

Abstract

Several vaccines are already produced using the baculovirus expression vector system (BEVS). This chapter describes methods for generating recombinant baculoviral DNA (also called bacmid) for cultivating Spodoptera frugiperda Sf-9 cells and producing a baculovirus stock from the recombinant bacmid and for producing a protein-based vaccine with the BEVS in a stirred tank reactor.

Keywords: Antigen production; Cell cultivation; Dielectric spectroscopy; Online process monitoring; Process analytical technology; Sf-9 cells; Spodoptera frugiperda.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens / biosynthesis*
  • Antigens / genetics*
  • Antigens / isolation & purification
  • Baculoviridae / genetics*
  • Batch Cell Culture Techniques*
  • Bioreactors*
  • Cell Culture Techniques
  • Cloning, Molecular
  • Gene Expression
  • Genetic Engineering
  • Genetic Vectors / genetics*
  • Recombinant Proteins*
  • Sf9 Cells
  • Transfection
  • Workflow

Substances

  • Antigens
  • Recombinant Proteins