Several vaccines are already produced using the baculovirus expression vector system (BEVS). This chapter describes methods for generating recombinant baculoviral DNA (also called bacmid) for cultivating Spodoptera frugiperda Sf-9 cells and producing a baculovirus stock from the recombinant bacmid and for producing a protein-based vaccine with the BEVS in a stirred tank reactor.
Keywords: Antigen production; Cell cultivation; Dielectric spectroscopy; Online process monitoring; Process analytical technology; Sf-9 cells; Spodoptera frugiperda.