Biological cells and coupled electro-mechanical effects: The role of organelles, microtubules, and nonlocal contributions

Abstract

Biological cells are exposed to a variety of mechanical loads throughout their life cycles that eventually play an important role in a wide range of cellular processes. The understanding of cell mechanics under the application of external stimuli is important for capturing the nuances of physiological and pathological events. Such critical knowledge will play an increasingly vital role in modern medical therapies such as tissue engineering and regenerative medicine, as well as in the development of new remedial treatments. At present, it is well known that the biological molecules exhibit piezoelectric properties that are of great interest for medical applications ranging from sensing to surgery. In the current study, a coupled electro-mechanical model of a biological cell has been developed to better understand the complex behaviour of biological cells subjected to piezoelectric and flexoelectric properties of their constituent organelles under the application of external forces. Importantly, a more accurate modelling paradigm has been presented to capture the nonlocal flexoelectric effect in addition to the linear piezoelectric effect based on the finite element method. Major cellular organelles considered in the developed computational model of the biological cell are the nucleus, mitochondria, microtubules, cell membrane and cytoplasm. The effects of variations in the applied forces on the intrinsic piezoelectric and flexoelectric contributions to the electro-elastic response have been systematically investigated along with accounting for the variation in the coupling coefficients. In addition, the effect of mechanical degradation of the cytoskeleton on the electro-elastic response has also been quantified. The present studies suggest that flexoelectricity could be a dominant electro-elastic coupling phenomenon, exhibiting electric fields that are four orders of magnitude higher than those generated by piezoelectric effects alone. Further, the output of the coupled electro-mechanical model is significantly dependent on the variation of flexoelectric coefficients. We have found that the mechanical degradation of the cytoskeleton results in the enhancement of both the piezo and flexoelectric responses associated with electro-mechanical coupling. In general, our study provides a framework for more accurate quantification of the mechanical/electrical transduction within the biological cells that can be critical for capturing the complex mechanisms at cellular length scales.

Keywords: Cell model; Electro-mechanical coupling; Finite element modelling; Flexoelectricity; Medical therapies; Microtubules; Piezoelectricity.