QTL mapping and marker identification for sex determination in the ridgetail white prawn, Exopalaemon carinicauda

Jianjian Lv; Xuan Lu; Xingbin Ti; Ping Liu; Jitao Li; Jian Li

doi:10.1016/j.ygeno.2020.09.037

QTL mapping and marker identification for sex determination in the ridgetail white prawn, Exopalaemon carinicauda

Genomics. 2020 Nov;112(6):5240-5247. doi: 10.1016/j.ygeno.2020.09.037. Epub 2020 Sep 18.

Authors

Jianjian Lv¹, Xuan Lu², Xingbin Ti², Ping Liu¹, Jitao Li³, Jian Li⁴

Affiliations

¹ Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, P.R. China, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
² Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, P.R. China, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
³ Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, P.R. China, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. Electronic address: lijt@ysfri.ac.cn.
⁴ Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, P.R. China, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. Electronic address: lijian@ysfri.ac.cn.

PMID: 32956845
DOI: 10.1016/j.ygeno.2020.09.037

Abstract

Sex determination is an important and intriguing research topic in the field of evolutionary and developmental biology. Quantitative trait locus (QTL) mapping for sex is helpful in clarifying the sex determination system of species. In this study, a second high-resolution genetic linkage map was constructed for the ridgetail white prawn, Exopalaemon carinicauda, which included 9280 markers, covering 99.98% of the complete genome. Based on the linkage map, a highly significant sex-related QTL was first mapped to a single linkage group (LG3, LOD > 55.6). Fifty-two markers in the QTL region were significantly associated with sex (p ≤ 10^-40), of which heterogametic genotypes in females supported the ZW sex determination mechanism. Six markers were verified to be significantly associated with sex in the wild population. Some sex-related genes were identified, including phospholipase D, protein kinase shaggy, and longitudinals lacking protein. These results inform our understanding of the mechanisms of sex determination in E. carinicauda.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chromosome Mapping
Female
Genetic Linkage
Genetic Markers
Genotyping Techniques
Male
Palaemonidae / genetics*
Polymorphism, Single Nucleotide
Quantitative Trait Loci*
Sex Determination Processes*

Substances

Genetic Markers