Hematopoiesis is a complex, orderly and conserved developmental process, coordinated by multiple factors including transcription factors and signaling pathways. Dysregulation of any of these factors may cause developmental or functional defects in the blood system, leading to the pathogenesis of blood diseases. Zebrafish hematopoiesis and the underlying molecular mechanisms are highly conserved with those in mammals. The use of zebrafish to recapitulate abnormal changes in pathogenic factors can build models of related blood diseases, thus providing powerful tools for exploring the molecular mechanisms of pathogenesis and progression, visualization of tumorigenesis and high-throughput chemical screening. In this review, we summarize the zebrafish models of blood diseases and their applications. These disease models not only help to improve our understanding of the pathophysiology of the blood system and the molecular mechanisms on pathogeneses of blood diseases, but also provide new ideas for the treatment of clinically relevant hematological malignancies.
血液发育是一个复杂有序且保守的过程,由多种转录因子和信号通路协同调控,任何环节的失调都可能引起血液系统发育或功能缺陷,导致血液疾病的发生。斑马鱼(Danio rerio)造血过程及分子调控机制与哺乳动物高度保守。应用斑马鱼模拟致病因子的异常变化构建相关血液疾病模型,为探究疾病发生机制、肿瘤发生发展可视化研究及高通量化学筛选提供了有力的工具。本文概述了斑马鱼血液疾病模型及其应用,这些疾病模型不仅有助于完善对血液系统病理生理学、血液疾病发生分子机制的认识,也为临床相关恶性血液疾病的治疗提供了新思路。.
Keywords: animal model; blood diseases; chemical screens; zebrafish.