Immobilized Pseudomonas aeruginosa beads with alginate and biochar as composite carriers and a nonionic surfactant (TX100) as degradation promoter were prepared by the gel embedding method. The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively. The addition of TX100 can simultaneously promote biochar sorption of PAHs and PAH degradation by P. aeruginosa. The removal ratio of acenaphthene was 24% higher for the TX100-facilitated immobilized bacterial beads than the beads in the absence of TX100. The surfactant-facilitated immobilized bacterial beads can thoroughly remove PAHs in wastewater under the conditions of 10~50 °C, pH 2.5~10.5, and less than 0.2 mol/L NaCl. The immobilized bacterial beads are suitable for continuous-flow reactors, and 2-mm-diameter beads will achieve better application results than larger beads. The new immobilized material can be widely used in various wastewater treatment reactors and in the in situ remediation of organic polluted water.
Keywords: Acenaphthene; Alginate embedding; Biochar; P. aeruginosa; Triton X-100; Wastewater treatment.