Abstract
Malaria-causing Plasmodium parasites traverse the mosquito midgut cells to establish infection at the basal side of the midgut. This dynamic process is a determinant of mosquito vector competence, yet the kinetics of the parasite migration is not well understood. Here we used transgenic mosquitoes of two Anopheles species and a Plasmodium berghei fluorescence reporter line to track parasite passage through the mosquito tissues at high spatial resolution. We provide new quantitative insight into malaria parasite invasion in African and Indian Anopheles species and propose that the mosquito complement-like system contributes to the species-specific dynamics of Plasmodium invasion.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anopheles / growth & development
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Anopheles / parasitology*
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Digestive System / parasitology*
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Female
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Host-Parasite Interactions*
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Malaria / parasitology
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Malaria / transmission*
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Mice
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Mosquito Vectors / pathogenicity*
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Plasmodium berghei / physiology*
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Species Specificity
Grants and funding
EAL received funding by EC FP7 Capacities Specific Programme Research Infrastructures ‘‘INFRAVEC” under grant agreement 228421. EAL received funding from the International Associate Laboratory (LIA - Laboratoire International Associé) grant from CNRS. JeS and PPG acknowledge the Structure fédérative de recherche santé François-Bonamy and the SERPICO team, are members of the national infrastructure "France BioImaging" supported by the ANR PIA1 (ANR-10-INBS-04). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.