Update on Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Identification of Filamentous Fungi

Laura S Wilkendorf; Edmée Bowles; Jochem B Buil; Henrich A L van der Lee; Brunella Posteraro; Maurizio Sanguinetti; Paul E Verweij

doi:10.1128/JCM.01263-20

Update on Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Identification of Filamentous Fungi

J Clin Microbiol. 2020 Nov 18;58(12):e01263-20. doi: 10.1128/JCM.01263-20. Print 2020 Nov 18.

Authors

Laura S Wilkendorf¹, Edmée Bowles¹, Jochem B Buil^{2

3}, Henrich A L van der Lee^{1

3}, Brunella Posteraro^{4

5}, Maurizio Sanguinetti^{4

6}, Paul E Verweij^{1

3}

Affiliations

¹ Radboud University Medical Center, Department of Medical Microbiology, Nijmegen, the Netherlands.
² Radboud University Medical Center, Department of Medical Microbiology, Nijmegen, the Netherlands jochem.buil@radboudumc.nl.
³ Center of Expertise in Mycology Radboudumc/CWZ, Nijmegen, the Netherlands.
⁴ Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy.
⁵ Dipartimento di Scienze Gastroenterologiche, Endocrino-Metaboliche e Nefro-Urologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy.
⁶ Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy.

Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based species identification has found its place in many clinical routine diagnostic laboratories over the past years, allowing significantly reduced turnaround times and high-precision results. With regard to MALDI-TOF MS for filamentous fungi, here, we discuss different approaches for sample processing and growth conditions before analysis. In particular, we review the performances of different commercially available databases as well as the potential of complementary (self-constructed) in-house databases.

Keywords: Biotyper; Bruker; MALDI-TOF MS; Vitek; filamentous fungi; identification; in-house database.

Publication types

Review

MeSH terms

Clinical Laboratory Services*
Fungi*
Humans
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization