Characterization and digestion features of a novel polysaccharide-Fe(III) complex as an iron supplement

Linlin Wang; Lilong Wang; Changyu Su; Chengrong Wen; Yue Gong; Ying You; Jun Zhao; Yanhui Han; Shuang Song; Hang Xiao

doi:10.1016/j.carbpol.2020.116812

Characterization and digestion features of a novel polysaccharide-Fe(III) complex as an iron supplement

Carbohydr Polym. 2020 Dec 1:249:116812. doi: 10.1016/j.carbpol.2020.116812. Epub 2020 Jul 29.

Authors

Linlin Wang¹, Lilong Wang¹, Changyu Su¹, Chengrong Wen¹, Yue Gong¹, Ying You², Jun Zhao¹, Yanhui Han³, Shuang Song⁴, Hang Xiao³

Affiliations

¹ National Engineering Research Center of Seafood, Collaborative Innovation Center of Seafood Deep Processing, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National & Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, PR China.
² National Engineering Research Center of Seafood, Collaborative Innovation Center of Seafood Deep Processing, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National & Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, PR China; College of Food Science and Engineering, Jilin Agricultural University, Changchun, PR China.
³ Department of Food Science, University of Massachusetts Amherst, Amherst, MA, 01003, USA.
⁴ National Engineering Research Center of Seafood, Collaborative Innovation Center of Seafood Deep Processing, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National & Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, PR China; Department of Food Science, University of Massachusetts Amherst, Amherst, MA, 01003, USA. Electronic address: songs1008@163.com.

PMID: 32933660
DOI: 10.1016/j.carbpol.2020.116812

Abstract

Using a sulfated polysaccharide from the gonad of pacific abalone (AGSP), a novel polysaccharide-Fe(III) complex, AGSP-Fe(III), was synthesized, and it was characterized by a series of methods including ultraviolet-visible (UV-vis) spectroscopy, Fourier transform-infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, scanning electron microscopy (SEM), and atomic force microscopy (AFM). The results showed that AGSP-Fe(III) was formed by the Fe-O bond between sulfate, carboxyl, and hydroxyl groups in AGSP and Fe, its particle size reached a maximum of 200 nm after aggregating, and its surface morphology presented relatively regular columnar or spherical. Moreover, the iron release of AGSP-Fe(III) during simulated gastrointestinal digestion was exhibited, and its good iron supplementary efficiency was also shown using Caco-2 cells. In addition, compared to FeCl₃, AGSP-Fe(III) showed better solubility and stability in the presence of polyphenol/trypsin. The present study demonstrated the potential of AGSP-Fe(III) as a novel iron supplement.

Keywords: Caco-2 cells; In vitro digestion; Iron supplement; Polyphenol; Polysaccharide-Fe(III) complex.