A review of co-culture models to study the oral microenvironment and disease

Sophie E Mountcastle; Sophie C Cox; Rachel L Sammons; Sara Jabbari; Richard M Shelton; Sarah A Kuehne

doi:10.1080/20002297.2020.1773122

A review of co-culture models to study the oral microenvironment and disease

J Oral Microbiol. 2020 Jun 4;12(1):1773122. doi: 10.1080/20002297.2020.1773122.

Authors

Sophie E Mountcastle^{1

2}, Sophie C Cox³, Rachel L Sammons², Sara Jabbari^{4

5}, Richard M Shelton², Sarah A Kuehne^{2

5}

Affiliations

¹ EPSRC Centre for Doctoral Training in Physical Sciences for Health, University of Birmingham, Birmingham, UK.
² School of Dentistry, University of Birmingham, Birmingham, UK.
³ School of Chemical Engineering, University of Birmingham, Birmingham, UK.
⁴ School of Mathematics, University of Birmingham, Birmingham, UK.
⁵ Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK.

Abstract

Co-cultures allow for the study of cell-cell interactions between different eukaryotic species or with bacteria. Such an approach has enabled researchers to more closely mimic complex tissue structures. This review is focused on co-culture systems modelling the oral cavity, which have been used to evaluate this unique cellular environment and understand disease progression. Over time, these systems have developed significantly from simple 2D eukaryotic cultures and planktonic bacteria to more complex 3D tissue engineered structures and biofilms. Careful selection and design of the co-culture along with critical parameters, such as seeding density and choice of analysis method, have resulted in several advances. This review provides a comparison of existing co-culture systems for the oral environment, with emphasis on progression of 3D models and the opportunity to harness techniques from other fields to improve current methods. While filling a gap in navigating this literature, this review ultimately supports the development of this vital technique in the field of oral biology.

Keywords: 3D-in vitro models; Co-culture; NC3Rs; biofilms; oral cavity; oral disease.

Publication types

Review

Grants and funding

EPSRC provided funding through a studentship at the Centre for Doctoral Training in Physical Sciences for Health [EP/L016346/1]. The funding body had no involvement in the writing of the manuscript and in the decision to submit the manuscript for publication.