Growth performance and nitrogen allocation within leaves of two poplar clones after exponential and conventional nitrogen applications

Abstract

Populus species are fast growing with high N requirements; an optimum level of fertilization is necessary for high seedling quality and subsequent plantation productivity. In this study, the morphological and physiological responses of two poplar clones (XH and BL3) to exponential and conventional N dosages were investigated, with a specific focus on leaf traits, the photorespiratory N cycle, and the interconversion of amino acids within leaves. Results show that shoot height and leaf number exponentially increased with plant growth. Leaf area, chlorophyll concentration, and net photosynthetic rate significantly increased for both clones during N fertilization, with a significant difference only in leaf area of clone XH between exponential and conventional dosages. Leaf concentrations of free amino acids and soluble sugars were not different but soluble proteins and fatty acids were significantly different for clone XH between N dosages; the amino acids glutamate, alanine, and aspartic acid concentrations increased in exponentially fertilized seedlings compared to controls. Amino acids, including the composition concentration and activity of glutamic-oxalacetic and -pyruvic transaminase, and soluble sugars were significantly higher for clone BL3 in fertilized seedlings. Photorespiration (glycine and glycolate oxidase) and glutathione redox (oxidized glutathione) were affected by fertilization. The activities of key enzymes (glycolate oxidase, catalase, and γ-glutamate cysteine ligase) involved in photorespiration and glutathione metabolism were lower for clone XH with exponential fertilization. Phenylalanine catabolism was influenced by fertilization and the interaction, clone × fertilization, showing accumulation of phenylalanine and tyrosine but decreases in phenylalanine ammonialyase activity and flavonoid concentrations in leaves of fertilized seedlings. The results indicate that leaf area and the interconversion of amino acids through deamidation/transamination are key regulatory hubs in poplar acclimation to soil N availability.

Keywords: Exponential fertilization; Glutathione redox; Leaf area; Phenylalanine catabolism; Photorespiration; Poplar; Transamination.