New cannabinoid receptor antagonists as pharmacological tool

Bioorg Med Chem. 2020 Oct 1;28(19):115672. doi: 10.1016/j.bmc.2020.115672. Epub 2020 Jul 29.

Abstract

Synthesis and pharmacological evaluation of a new series of cannabinoid receptor antagonists of indazole ether derivatives have been performed. Pharmacological evaluation includes radioligand binding assays with [3H]-CP55940 for CB1 and CB2 receptors and functional activity for cannabinoid receptors on isolated tissue. In addition, functional activity of the two synthetic cannabinoids antagonists 18 (PGN36) and 17 (PGN38) were carried out in the osteoblastic cell line MC3T3-E1 that is able to express CB2R upon osteogenic conditions. Both antagonists abolished the increase in collagen type I gene expression by the well-known inducer of bone activity, the HU308 agonist. The results of pharmacological tests have revealed that four of these derivatives behave as CB2R cannabinoid antagonists. In particular, the compounds 17 (PGN38) and 18 (PGN36) highlight as promising candidates as pharmacological tools.

Keywords: Antagonist; Bone disease; Cannabinoid receptors; Indazole ether; Pharmacological tool.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Cannabinoid Receptor Antagonists / chemical synthesis
  • Cannabinoid Receptor Antagonists / chemistry
  • Cannabinoid Receptor Antagonists / pharmacology*
  • Cannabinoids / chemistry
  • Cannabinoids / pharmacology*
  • Dose-Response Relationship, Drug
  • Ethers / chemical synthesis
  • Ethers / chemistry
  • Ethers / pharmacology*
  • Indazoles / chemical synthesis
  • Indazoles / chemistry
  • Indazoles / pharmacology*
  • Mice
  • Molecular Structure
  • Receptors, Cannabinoid / metabolism*
  • Structure-Activity Relationship

Substances

  • Cannabinoid Receptor Antagonists
  • Cannabinoids
  • Ethers
  • Indazoles
  • Receptors, Cannabinoid
  • HU 308