Expression of RUNX2 and Osterix in Rat Mesenchymal Stem Cells during Culturing in Osteogenic-Conditioned Medium

L A Pokrovskaya; S V Nadezhdin; E V Zubareva; Yu E Burda; E S Gnezdyukova

doi:10.1007/s10517-020-04931-5

Expression of RUNX2 and Osterix in Rat Mesenchymal Stem Cells during Culturing in Osteogenic-Conditioned Medium

Bull Exp Biol Med. 2020 Aug;169(4):571-575. doi: 10.1007/s10517-020-04931-5. Epub 2020 Sep 10.

Authors

L A Pokrovskaya¹, S V Nadezhdin², E V Zubareva³, Yu E Burda⁴, E S Gnezdyukova³

Affiliations

¹ National Research Tomsk State University, Tomsk, Russia.
² Research Laboratory "Cellular, Assisted Reproductive, and DNA Technologies", Belgorod State National Research University, Belgorod, Russia. nadezhdin@bsu.edu.ru.
³ Research Laboratory "Cellular, Assisted Reproductive, and DNA Technologies", Belgorod State National Research University, Belgorod, Russia.
⁴ Innovation Centre "Biruch - New Technologies", Alekseevka, Belgorod region, Russia.

PMID: 32910396
DOI: 10.1007/s10517-020-04931-5

Abstract

We studied the expression of transcription factors RUNX2 and Osterix after addition of a concentrate of osteogenic-conditioned medium to the culture medium for osteogenic differentiation of mesenchymal stem cells (MSC). The obtained concentrate of osteogenic-conditioned medium containing a complex of bioactive substances with a molecular weight >10 kDa provided MSC differentiation into osteoblasts, which was confirmed by high level of expression of transcription factors RUNX2 and Osterix in comparison with the negative control. The highest expression of transcription factor Osterix was revealed on day 14 of MSC culturing in the presence of osteogenic supplement StemPro (positive control) and the studied concentrate of osteogenic-conditioned medium.

Keywords: differentiation; mesenchymal stem cells; osteogenic-conditioned medium; osteoinduction.

MeSH terms

Animals
Ascorbic Acid / pharmacology
Biomarkers / metabolism
Bone Marrow Cells / cytology
Bone Marrow Cells / drug effects
Bone Marrow Cells / metabolism
Cell Differentiation / drug effects
Core Binding Factor Alpha 1 Subunit / genetics*
Core Binding Factor Alpha 1 Subunit / metabolism
Culture Media, Conditioned / chemistry
Culture Media, Conditioned / pharmacology*
Dexamethasone / pharmacology
Gene Expression
Glycerophosphates / pharmacology
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / drug effects*
Mesenchymal Stem Cells / metabolism
Osteoblasts / cytology
Osteoblasts / drug effects*
Osteoblasts / metabolism
Osteogenesis / drug effects*
Osteogenesis / genetics
Primary Cell Culture
Rats
Rats, Wistar
Transcription Factors / genetics*
Transcription Factors / metabolism

Substances

Biomarkers
Core Binding Factor Alpha 1 Subunit
Culture Media, Conditioned
Glycerophosphates
Runx2 protein, rat
Sp7 protein, rat
Transcription Factors
Dexamethasone
Ascorbic Acid
beta-glycerophosphoric acid