Abstract
Using multiplex analysis, we performed a comparative study of cytokine and growth factor production by human umbilical cord tissue-derived multipotent mesenchymal stromal cells (UC-MSC) cultured under standard conditions and in the presence of human umbilical cord blood serum (UCBS). It was found that the secretion of most studied molecules, including well-known inductors of regeneration HGF, G-CSF, GM-CSF, and VEGF by UCMSC considerably increased in the presence of 5% UCBS. The use of UCBS allows not only obtaining xenogenic-free cellular and cell-free therapeutic products, but also increasing the secretion of most biologically active molecules capable of stimulating repair processes.
Keywords:
cord blood serum; cytokines; growth factors; multipotent mesenchymal stromal cells; umbilical cord.
MeSH terms
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Animals
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Cattle
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Culture Media / chemistry
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Culture Media / pharmacology*
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Fetal Blood / chemistry*
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Fetus
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Gene Expression
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Granulocyte Colony-Stimulating Factor / genetics
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Granulocyte Colony-Stimulating Factor / metabolism
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Granulocyte-Macrophage Colony-Stimulating Factor / genetics
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Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
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Hepatocyte Growth Factor / genetics
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Hepatocyte Growth Factor / metabolism
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Humans
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Interleukins / genetics
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Interleukins / metabolism
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Mesenchymal Stem Cells / cytology
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Mesenchymal Stem Cells / drug effects*
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Mesenchymal Stem Cells / metabolism
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Primary Cell Culture
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Serum Albumin, Bovine / chemistry
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Serum Albumin, Bovine / pharmacology*
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Umbilical Cord / chemistry*
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Umbilical Cord / cytology
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Umbilical Cord / metabolism
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Vascular Endothelial Growth Factor A / genetics
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Vascular Endothelial Growth Factor A / metabolism
Substances
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Culture Media
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HGF protein, human
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Interleukins
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VEGFA protein, human
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Vascular Endothelial Growth Factor A
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Granulocyte Colony-Stimulating Factor
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Serum Albumin, Bovine
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Hepatocyte Growth Factor
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Granulocyte-Macrophage Colony-Stimulating Factor