Administration of Bacillus Amyloliquefaciens and Saccharomyces Cerevisiae as Direct-Fed Microbials Improves Intestinal Microflora and Morphology in Broiler Chickens

Po-Yun Teng; Chi-Huan Chung; Yun-Peng Chao; Chung-Jen Chiang; Shen-Chang Chang; Bi Yu; Tzu-Tai Lee

doi:10.2141/jpsa.0160069

Administration of Bacillus Amyloliquefaciens and Saccharomyces Cerevisiae as Direct-Fed Microbials Improves Intestinal Microflora and Morphology in Broiler Chickens

J Poult Sci. 2017 Apr 25;54(2):134-141. doi: 10.2141/jpsa.0160069.

Authors

Po-Yun Teng¹, Chi-Huan Chung¹, Yun-Peng Chao^{2

3}, Chung-Jen Chiang⁴, Shen-Chang Chang⁵, Bi Yu¹, Tzu-Tai Lee¹

Affiliations

¹ Department of Animal Science, National Chung Hsing University, Taichung, 402, Taiwan.
² Department of Chemical Engineering, Feng Chia University, Taichung, 402, Taiwan.
³ Department of Health and Nutrition Biotechnology, Asia University, Taichung 413, Taiwan.
⁴ Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, 404, Taiwan.
⁵ Kaosiung Animal Propagation Station, Livestock Research Institute, Council of Agriculture, Pingtung 912, Taiwan.

Abstract

This study was conducted to investigate the effects of Bacillus amyloliquefaciens (BA) and Saccharomyces cerevisiae (SC) as directed-fed microbials on performance, intestinal microflora, and intestinal morphology in broiler chickens. A total of four hundred one-day-old broiler chickens were randomly divided into 16 pens of 25 chickens each, and every treatment had 4 replicated pens with two pens of males and females respectively. A formulated corn-soybean meal based control diets and experimental diets, including 0.1% BA (1×10⁷ colony-forming units (CFU)/kg), the mixture of 0.05% BA (5×10⁶ CFU/kg) and 0.05% SC (5×10⁶ CFU/kg), and 10 ppm antibiotic (avilamycin), were fed for 5 weeks. The results showed no significant difference in the growth performance among all treatments. Supplementation of the mixture of BA and SC increased acetate and propionate and decreased the E. coli in ceca compared to control and antibiotic treatment. The treatments with antibiotic, BA, and the mixture of BA and SC compared to control treatment increased villus height / crypt depth ratio and decreased ammonia in excreta. In addition, supplementation of BA and the mixture of BA and SC compared to antibiotic treatment increased serum high-density lipoprotein, and decreased serum glutamic-oxaloacetic transaminase, respectively. In conclusion, supplementation of the mixture of BA and SC was better than added BA only, and the mixed probiotics product could potentially alter the use of avilamycin in broiler diets.

Keywords: Bacillus amyloliquefaciens; Saccharomyces cerevisiae; antibiotic; broilers.

2017, Japan Poultry Science Association.